Anti-Hepatitis C Non structural 5B (NS5B) [1A4] Antibody

This mouse monoclonal antibody was raised against recombinant GST-nonstructural 5B protein (NS5B), aa 1-346, Hepatitis C virus (HCV) genotype 2a strain fusion protein and recognizes NS5B of HCV.

Highlights:

  • Reacts with nonstructural 5B protein (NS5B) of HCV
  • Suitable for Western Blot and Immunofluorscence applications

Hepatitis C is an infectious disease caused by the hepatitis C virus (HCV) that primarily affects the liver. HCV encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV's RNA dependant RNA polymerase and is critical for viral replication.

From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.

Catalog Number Product Size AVAILABILITY Price Qty
ESG010
Anti-Hepatitis C Non structural 5B (NS5B) [1A4] Antibody
5mL supernatant 3-5 weeks
Regular Price:$250.00
Specifications

Product Type: Antibody
Antigen: Nonstructural 5B protein (NS5B) of Hepatitis C virus
Molecular Weight: 65 kDa
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 1A4
Reactivity: Hepatitis C virus
Immunogen: Recombinant GST-NS5B (aa 1-346, HCV genotype 2a strain) fusion protein
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IF
Storage: 80C
Shipped: Dry Ice

Data

Immunofluorescence

Huh7.5 cells were mock infected or infected with HCV were fixed using 4% formaldehyde solution at 2 days post infection. Immunofluorescence assay was performed using purified 1A4 at the 20ug/ml. DAPI was used to stain the nucleus.

Western Blot

Genes encoding full length NS5B proteins (with a N-terminal flag-tag) from 3 HCV genotypes, namely 1b, 2a, and 3a, were over-expressed in 293FT cells, followed by lysis of cells in RIPA buffer, Mock refers to cells transfected with empty vector only. SDS-PAGE separation and transfer by Western blotting. Membranes were probed with purified mAbs at 2ug/ml.

References
  1. Jude J Aweya; Ching Wooen Sze; Anthony Bayega; Nur Khairiah Mohd-Ismail; Lin Deng; Hak Hotta; Yee-Joo Tan. NS5B induces up-regulation of the BH3-only protein, BIK, essential for the Hepatitis C virus RNA replication and viral release. Virology. 2015 Jan 1;474:41-51.
  2. If you publish research with this product, please let us know so we can cite your paper.

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