Anti-Hepatitis C Non structural 5B (NS5B) [1A4] Antibody
![Anti-Hepatitis C Non structural 5B (NS5B) [1A4] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1706_2001202001265817050.jpg "Anti-Hepatitis C Non structural 5B (NS5B) [1A4] Antibody")
This mouse monoclonal antibody was raised against recombinant GST-nonstructural 5B protein (NS5B), aa 1-346, Hepatitis C virus (HCV) genotype 2a strain fusion protein and recognizes NS5B of HCV.
Highlights:
- Reacts with nonstructural 5B protein (NS5B) of HCV
- Suitable for Western Blot and Immunofluorscence applications
Hepatitis C is an infectious disease caused by the hepatitis C virus (HCV) that primarily affects the liver. HCV encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV's RNA dependant RNA polymerase and is critical for viral replication.
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
This mouse monoclonal antibody was raised against recombinant GST-nonstructural 5B protein (NS5B), aa 1-346, Hepatitis C virus (HCV) genotype 2a strain fusion protein and recognizes NS5B of HCV.
Highlights:
- Reacts with nonstructural 5B protein (NS5B) of HCV
- Suitable for Western Blot and Immunofluorscence applications
Hepatitis C is an infectious disease caused by the hepatitis C virus (HCV) that primarily affects the liver. HCV encodes a polyprotein that is cleaved into at least 10 viral proteins namely E1, E2, core, p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. Non-structural 5B protein (NS5B) is HCV's RNA dependant RNA polymerase and is critical for viral replication.
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
| Product Type: | Antibody |
| Antigen: | Nonstructural 5B protein (NS5B) of Hepatitis C virus |
| Accession ID: | D3KY46 |
| Molecular Weight: | 65 kDa |
| Isotype: | IgG2a |
| Clonality: | Monoclonal |
| Clone Name: | 1A4 |
| Reactivity: | Hepatitis C virus |
| Immunogen: | Recombinant GST-NS5B (aa 1-346, HCV genotype 2a strain) fusion protein |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | WB, IF |
| Storage: | 80C |
| Shipped: | Cold Packs |
Immunofluorescence
Huh7.5 cells were mock infected or infected with HCV were fixed using 4% formaldehyde solution at 2 days post infection. Immunofluorescence assay was performed using purified 1A4 at the 20ug/ml. DAPI was used to stain the nucleus.
Western Blot
Genes encoding full length NS5B proteins (with a N-terminal flag-tag) from 3 HCV genotypes, namely 1b, 2a, and 3a, were over-expressed in 293FT cells, followed by lysis of cells in RIPA buffer, Mock refers to cells transfected with empty vector only. SDS-PAGE separation and transfer by Western blotting. Membranes were probed with purified mAbs at 2ug/ml.
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
- Jude J Aweya; Ching Wooen Sze; Anthony Bayega; Nur Khairiah Mohd-Ismail; Lin Deng; Hak Hotta; Yee-Joo Tan. NS5B induces up-regulation of the BH3-only protein, BIK, essential for the Hepatitis C virus RNA replication and viral release. Virology. 2015 Jan 1;474:41-51.
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