Mocha cells are a rat cochlear microglial cell line. They were developed from an immunomagnetically enriched population of CD11b+ cells from P3 rat cochlea and immortalized with the 12S E1A gene of adenovirus in a replication-incompetent retroviral vector.
Microglia are a unique lineage of mononuclear phagocytic cells that play an essential role in development, immunological defense and in- flammatory processes in the central nervous system. Microglia account for 10-15% of all cells found within the brain. As the resident macrophage cells, they act as the first and main form of active immune defence in the central nervous system (CNS).
From the laboratory of Gail M. Seigel, PhD, University at Buffalo.
|Product Type:||Cell Line|
|Name:||Mocha (short for microglia of the cochlea)|
|Cell Type:||Microglia of the cochlea|
|Organism:||P3 Sprague-Dawley rat|
|Morphology:||Mixed microglial (both amoeboid and partially ramified)|
|Subculturing:||Cells are adherent with doubling time of about 24 hours, Passage 3X per week with 0.125% trypsin-EDTA|
|Growth Conditions:||DMEM with 10% calf serum at 37C in 5% CO2|
|Cryopreservation:||DMEM/10% calf serum with 10% DMSO|
|Comments:||Mocha cells are resistant to neomycin/G418|
View Dr. Seigel's presentation describing the Mocha cell line here.