Marek's Disease Spleen Cell Line (MDCC-UA53)
MDCC (Marek's disease cell culture) UA53 cells were established from a spleen lymphoma induced by the TK strain of Marek's disease virus, a very virulent + MDV (vv+MDV).
Highlights:
- Established from TK + vaccine strain HVT-inoculated chickens and showed an abnormal immunophenotype (no TCR, CD3, CD4 or CD8 surface expression)
- Cells used to study the interaction of a vv+MDV with the chicken immune system
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
MDCC (Marek's disease cell culture) UA53 cells were established from a spleen lymphoma induced by the TK strain of Marek's disease virus, a very virulent + MDV (vv+MDV).
Highlights:
- Established from TK + vaccine strain HVT-inoculated chickens and showed an abnormal immunophenotype (no TCR, CD3, CD4 or CD8 surface expression)
- Cells used to study the interaction of a vv+MDV with the chicken immune system
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
Specifications
| Product Type: | Cell Line |
| Cell Type: | Chicken T-cells (CD3-,CD4-, CD8-, TCR2-, TCR-, CD28hi, CD30hi, CD44hi, CD164hi, MHC-IILO) |
| Morphology: | non-adherent, pleomorphic, lymphoblastoid |
| Source: | Spleen lymphoma |
| Organism: | Chicken, Gallus gallus, (SPF single-comb white leghorn) |
| Biosafety Level: | BSL-1 |
| Subculturing: | 1:5 to 1:10 every 2- 3 days |
| Growth Conditions: | Established Iscove's modified Dulbecco minimal essential medium (IMDMEM) supplemented with 20% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X nonessential amino acids, 1X insulin/transferrin/selenium, 1X Pen/Strept/Neo, 1X fungizone. Adapted to: DMEM, high glucose, 10% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone |
| Cryopreservation: | 90% FBS/10% DMSO |
| Comments: | Grow at 41C, 5% CO2. When actively growing, double every 12 - 24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
Provider
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
References
- Santin, E, CE Shamblin, JT Prigge, V Arumugaswami, RL Dienglewicz, and MS Parcells. 2006. Examination of the effect of a naturally occurring mutation in glycoprotein L on the Marek's disease virus pathogenesis. Avian Dis. 50:96-103.
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