Marek's Disease Kidney Cell Line (MDCC-UA22)
MDCC (Marek's disease cell culture) UA22 cells were established from a kidney-localized lymphoma using RB-1B-pp38smGFP, a recombinant Marek's disease virus (MDV), strain RB-1B, in which the soluble modified derivative of the green fluorescent protein (smGFP) was inserted downstream of, but not directly fused to, the pp38 protein of MDV.
Highlights:
- Expression of GFP is tightly controlled in these cells, with >1 % spontaneously expressing GFP
- Expression is induced during reactivation of the MDV from latency, therefore cells are ideal for studying the early events of reactivation
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
MDCC (Marek's disease cell culture) UA22 cells were established from a kidney-localized lymphoma using RB-1B-pp38smGFP, a recombinant Marek's disease virus (MDV), strain RB-1B, in which the soluble modified derivative of the green fluorescent protein (smGFP) was inserted downstream of, but not directly fused to, the pp38 protein of MDV.
Highlights:
- Expression of GFP is tightly controlled in these cells, with >1 % spontaneously expressing GFP
- Expression is induced during reactivation of the MDV from latency, therefore cells are ideal for studying the early events of reactivation
Marek's disease is a highly contagious viral neoplastic disease in chickens. Marek's disease is caused by an alphaherpesvirus known as 'Marek's disease virus' (MDV) or Gallid herpesvirus 2 (GaHV-2). The disease is characterized by the presence of T cell lymphoma as well as infiltration of nerves and organs by lymphocytes.
From the laboratory of Mark S. Parcells, PhD, University of Delaware.
| Product Type: | Cell Line |
| Cell Type: | Chicken T-cells (CD4+, TCR2+, CD3lo, CD28lo, MHC-IIhi) |
| Organism: | Chicken, Gallus gallus, (SPF single-comb white leghorn) |
| Source: | Kidney lymphoma |
| Morphology: | non-adherent, pleomorphic, lymphoblastoid |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | Established Iscove's modified Dulbecco minimal essential medium (IMDMEM) supplemented with 20% FBS,10% chicken serum, 10% tryptose-phosphate broth, 4 mM L-glutamine, 2 µM 2-mercaptoethanol, 1X nonessential amino acids, 1X insulin/transferrin/selenium, 1X Pen/Strept/Neo, 1X fungizone. Adapted to: RPM1-1640, 20% FBS, 4 mM L-glutamine, 1 X PSN, 1X fungizone |
| Subculturing: | 1:5 to 1:10 every 2 - 3 days |
| Cryopreservation: | 90% FBS/10% DMSO |
| Comments: | Grow at 41C, 5% CO2. When actively growing, double every 12 - 24 hrs. Grow at 5 x 105 to 2 x 107 cells/ml. |
| Storage: | Liquid nitrogen |
| Shipped: | Dry Ice |
- Prigge, JT, V. Majerciak, HD Hunt, RL Dienglewicz, and MS Parcells. 2004. Construction and characterization of Marek's disease viruses having green fluorescent protein expression tied directly or indirectly to phosphoprotein 38 expression. Avian Diseases 48:471-487.
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