Anti-Bacillus subtilis CodY Antibody
This rabbit polyclonal antibody was generated against purified Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY and is specific for CodY from B. subtilis.
Highlights:
- Reacts with CodY from B. subtilis
- Suitable for Western Blot applications
CodY (VEG286B) is a DNA-binding protein found in most low G+C Gram-positive bacteria that activates the expression of some genes that support rapid growth and represses the expression of many genes that are only induced as cells make the transition from rapid exponential growth to stationary phase and sporulation. It is activated as a DNA-binding protein by interaction with a branched-chain amino acid (isoleucine or leucine or valine) and GTP; these metabolites serve as indicators of nutritional sufficiency for rapid growth. As the concentrations of the metabolites drop, CodY loses its ability to bind DNA as either a negative or positive regulator.
From the laboratory of Abraham L Sonenshein, PhD, Tufts University.
This rabbit polyclonal antibody was generated against purified Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY and is specific for CodY from B. subtilis.
Highlights:
- Reacts with CodY from B. subtilis
- Suitable for Western Blot applications
CodY (VEG286B) is a DNA-binding protein found in most low G+C Gram-positive bacteria that activates the expression of some genes that support rapid growth and represses the expression of many genes that are only induced as cells make the transition from rapid exponential growth to stationary phase and sporulation. It is activated as a DNA-binding protein by interaction with a branched-chain amino acid (isoleucine or leucine or valine) and GTP; these metabolites serve as indicators of nutritional sufficiency for rapid growth. As the concentrations of the metabolites drop, CodY loses its ability to bind DNA as either a negative or positive regulator.
From the laboratory of Abraham L Sonenshein, PhD, Tufts University.
| Product Type: | Antibody |
| Antigen: | CodY |
| Accession ID: | P39779 |
| Molecular Weight: | 29,013 Da |
| Isotype: | IgG |
| Clonality: | Polyclonal |
| Reactivity: | Recognizes Bacillus subtilis CodY |
| Immunogen: | Purified Bacillus subtilis CodY |
| Species Immunized: | Rabbit |
| Buffer: | Whole serum |
| Tested Applications: | WB 1:5000 |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
- Ratnayake-Lecamwasam M, Serror P, Wong KW, Sonenshein AL. Bacillus subtilis CodY represses early-stationary-phase genes by sensing GTP levels. Genes Dev. 2001 May 1;15(9):1093-103.
- Basu A, Shields KE, Eickhoff CS, Hoft DF, Yap MN. Thermal and Nutritional Regulation of Ribosome Hibernation in Staphylococcus aureus. J Bacteriol. 2018 Nov 26;200(24). View Article
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