pCS2-SENP2(S)Neo Plasmid
pCS2-SENP2(S)Neo plasmid is a vector encoding the Senp2 gene for SUMO-specific protease 2 and the neomycin gene.
Highlights:
- Expresses the short isoform of SUMO-specific protease 2 (SENP2)
- Driven by a CMV promoter
- The SENP2 protein is fused to a myc tag
- Contains a phosphoglycerine kinase promoter region to drive the expression of a neomycin resistance gene for the purpose of selection
pCS2-SENP2(S) is a plasmid containing cDNA which encodes the short isoform of myc tagged SENP2. The expression is driven by CMV promoter. SENP2 encodes SUMO-specific protease 2 which is found in three alternatively spliced forms exhibiting differential subcellular localizations. Genetic inactivation of Senp2 in mice reveals its requirement in development of trophoblast stem cell niches and lineages during development of the placenta. The deletion of SENP2 in the neural progenitors results in neural degeneration mediated through modulation of Drp1-dependent mitochondrial morphogenesis.
From the laboratory of Wei Hsu, PhD, University of Rochester.
pCS2-SENP2(S)Neo plasmid is a vector encoding the Senp2 gene for SUMO-specific protease 2 and the neomycin gene.
Highlights:
- Expresses the short isoform of SUMO-specific protease 2 (SENP2)
- Driven by a CMV promoter
- The SENP2 protein is fused to a myc tag
- Contains a phosphoglycerine kinase promoter region to drive the expression of a neomycin resistance gene for the purpose of selection
pCS2-SENP2(S) is a plasmid containing cDNA which encodes the short isoform of myc tagged SENP2. The expression is driven by CMV promoter. SENP2 encodes SUMO-specific protease 2 which is found in three alternatively spliced forms exhibiting differential subcellular localizations. Genetic inactivation of Senp2 in mice reveals its requirement in development of trophoblast stem cell niches and lineages during development of the placenta. The deletion of SENP2 in the neural progenitors results in neural degeneration mediated through modulation of Drp1-dependent mitochondrial morphogenesis.
From the laboratory of Wei Hsu, PhD, University of Rochester.
| Product Type: | Plasmid |
| Gene/insert name: | SENP2-S |
| Accession ID: | Q9HC62 |
| Antibiotic Resistance: | Amp |
| Fusion Tag(s): | Myc tag |
| Grow in E. coli at 37 C: | Yes |
| Selectable markers: | Amp |
| Cloning Site 5': | Xho l |
| Cloning Site 3': | Xba I |
| Insert Size: | 1521 bp |
| Vector Backbone and Size: | 4352 Kb |
| High or low copy: | High |
| Storage: | -20C |
| Shipped: | Ambient temperature as liquid |
Notes:
CMV driven expression of SENP2 short isoform.
- Jiang, M., S.Y. Chiu, and W. Hsu, SUMO-specific protease 2 in Mdm2-mediated regulation of p53. Cell Death Differ, 2011. 18(6): p. 1005-15.
- Chiu, S.Y., N. Asai, F. Costantini, and W. Hsu, SUMO-Specific Protease 2 Is Essential for Modulating p53-Mdm2 in Development of Trophoblast Stem Cell Niches and Lineages. PLoS Biol, 2008. 6(12): p. e310.
- Fu, J., H.M. Yu, S.Y. Chiu, A.J. Mirando, E.O. Maruyama, J.G. Cheng, and W. Hsu, Disruption of SUMO-Specific Protease 2 Induces Mitochondria Mediated Neurodegeneration. PLoS Genet, 2014. 10(10): p. e1004579.
If you publish research with this product, please let us know so we can cite your paper.
