pCS2-SENP2(S)Neo Plasmid

pCS2-SENP2(S)Neo plasmid is a vector encoding the Senp2 gene for SUMO-specific protease 2 and the neomycin gene.

Highlights:

  • Expresses the short isoform of SUMO-specific protease 2 (SENP2)
  • Driven by a CMV promoter
  • The SENP2 protein is fused to a myc tag
  • Contains a phosphoglycerine kinase promoter region to drive the expression of a neomycin resistance gene for the purpose of selection

pCS2-SENP2(S) is a plasmid containing cDNA which encodes the short isoform of myc tagged SENP2. The expression is driven by CMV promoter. SENP2 encodes SUMO-specific protease 2 which is found in three alternatively spliced forms exhibiting differential subcellular localizations. Genetic inactivation of Senp2 in mice reveals its requirement in development of trophoblast stem cell niches and lineages during development of the placenta. The deletion of SENP2 in the neural progenitors results in neural degeneration mediated through modulation of Drp1-dependent mitochondrial morphogenesis.

From the laboratory of Wei Hsu, PhD, University of Rochester.

Catalog Number Product Size AVAILABILITY Price Qty
EUR311
pCS2-SENP2(S)Neo Plasmid
spotted on filter paper In stock
Regular Price:$68.00
Specifications

Product Type: Plasmid
Gene/insert name: SENP2-S
Antibiotic Resistance: Amp
Fusion Tag(s): Myc tag
Grow in E. coli at 37 C: Yes
Selectable markers: Amp
Cloning Site 5': Xho l
Cloning Site 3': Xba I
Insert Size: 1521 bp
Vector Backbone and Size: 4352 Kb
High or low copy: High
Storage: -20C
Shipped: Ambient temperature as liquid

Documentation

Notes:

CMV driven expression of SENP2 short isoform.

Provider
From the laboratory of Wei Hsu, PhD, University of Rochester.
References
  1. Jiang, M., S.Y. Chiu, and W. Hsu, SUMO-specific protease 2 in Mdm2-mediated regulation of p53. Cell Death Differ, 2011. 18(6): p. 1005-15.
  2. Chiu, S.Y., N. Asai, F. Costantini, and W. Hsu, SUMO-Specific Protease 2 Is Essential for Modulating p53-Mdm2 in Development of Trophoblast Stem Cell Niches and Lineages. PLoS Biol, 2008. 6(12): p. e310.
  3. Fu, J., H.M. Yu, S.Y. Chiu, A.J. Mirando, E.O. Maruyama, J.G. Cheng, and W. Hsu, Disruption of SUMO-Specific Protease 2 Induces Mitochondria Mediated Neurodegeneration. PLoS Genet, 2014. 10(10): p. e1004579.

If you publish research with this product, please let us know so we can cite your paper.

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