Anti-Beta-Arrestin [21-B1] Antibody

This mouse IgG1k monoclonal antibody was generated against human ß-arrestin-1 and recognizes human, rat, and hamster beta- arrestin 1 or beta-arrestin 2.

Highlights:

  • Recognizes human, rat, and hamster ß-arrestin-1 or ß-arrestin-2 (other species not tested)
  • Suitable for Western Blot and Immunoprecipitation applications

Beta-arrestins function in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein. Arrestin binding to the receptor blocks further G protein-mediated signaling and targets receptors for internalization, and redirects signaling to alternative G protein-independent pathways, such as ß-arrestin signaling. In addition to GPCRs, arrestins bind to other classes of cell surface receptors and a variety of other signaling proteins.

From the laboratory of Martin Oppermann, MD, University Medical Center Götingen.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGO011
Anti-Beta-Arrestin [21-B1] Antibody
100ug In stock
Regular Price:$306.00
On Sale:
Specifications

Product Type: Antibody
Antigen: Human and rat ß-arrestin-1; ß-arrestin-2
Isotype: IgG1k
Clonality: Monoclonal
Clone Name: 21-B1
Reactivity: Reacts equally well with (human, rat and hamster) beta- arrestin1 or beta-arrestin2; other species not tested
Immunogen: Human ß-arrestin-1
Species Immunized: Mouse (Balb/c)
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, IP
Storage: -20C
Shipped: Cold packs

Data

Immunoblots showing the specificity of anti beta-arrestin1/2 mAb 21-B1. Left: Cell lysates from HEK-293 cells overexpressing either rat beta-arrestin1 (A) or rat beta-arrestin2 (B) were probed with 21-B1 and two other mAb specific for beta-arrestin1 (7-F3 and 25-G10). Right: C5a-induced translocation of endogenous beta-arrestin1/2 from the cytosol (Cyt) to the plasma membrane (Mem) in the human myelo-monocytic cell line U937.

Provider
From the laboratory of Martin Oppermann, MD, University Medical Center Götingen.
References
  1. F. Hu?ttenrauch, B. Pollok-Kopp, M. Oppermann. G Protein- coupled receptor kinases promote phosphorylation and beta- arrestin-mediated internalization of CCR5 homo- and hetero- oligomers. J Biol Chem 280:37503 (2005)
  2. B. Pollok-Kopp, F. Hu?ttenrauch, S. Rethorn, M. Oppermann. Dynamics of protein kinase C -mediated phosphorylation of the complement C5a receptor on serine-334 (Submitted)

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