BHQ-O-5HT is a photoactivatable form of the neurotransmitter serotonin (5-HT) that is activated with either 1-photon excitation (1PE) at 368 nm or 2-photon excitation (2PE) at 740 nm in a biological preparation, such as cell culture, tissue slice, ex vivo brain, or whole animals.
Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. BHQ-O-5HT is a "caged" 5-HT molecule, that, upon photoactivation, releases 5-HT, enabling temporal control of the release of 5-HT within a biological system. BHQ-O-5HT depolarized sensory neurons when photolyzed in culture and in larval zebrafish comparable to that observed by 5-HT by itself. The quantum efficiency (Qu) of BHQ-O-5HT·2HBr for 1-photon photolysis is 0.40 at 365 nm in KMOPS buffer at pH 7.2. The 2-photon uncaging action cross-section (du) is 0.50 GM.
Part of The Investigator's Annexe program.
|Product Type:||Small Molecule|
|Name:||BHQ-O-5HT hydrogen bromide|
|Stability:||>6 months at -20C|
|Solubility:||>5mg in 100 mL KMOPS buffer (pH 7.2)|
|Spectral Information:||λmax= 371nm; ε= 4700 M-1cm-1, Qu= 0.4|
|Storage:||-20C; Protect from light|
Provided in salt form is for increased dry storage stability and easier handling.
Recommended dilution is 100uM at physiological pH. For best results, prepare solutions immediately prior to use. A stock solution in DMSO stored at -20C and in the dark has a reasonable shelf life. Once in aqueous enviornment, protect from light. Work under red light while handling the compounds before and during use.
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