NIH 2/4 Cell Line

The NIH 2/4 Cell Line allows for the direct study of the dynamics of single double-strand breaks (DSBs) and translocations in living mammalian cells in vivo.

NIH3T3 cells containing a copy of the unique 18 nucleotide ISceI restriction site flanked by an array of 256 copies of the lac-repressor binding site and by 96 copies of the tetracycline response element (LacOISceITetO array). This cell line does not express fluorescent repressors.

From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ENH101-FP
NIH 2/4 Cell Line
1 vial 4-6 weeks
Regular Price:$787.00
On Sale:

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Specifications

Product Type: Cell Line
Name: NIH 2/4
Cell Type: NIH3T3 mouse embryonic fibroblasts
Accession ID: CVCL_5I45
Organism: Mouse
Source: mouse embryo (NIH3T3 cells)
Morphology: Fibroblast
Biosafety Level: BSL1
Subculturing: Split 1 to 3 when ?90% confluency
Growth Conditions: High-glucose DMEM, 10-15% FBS, P/S, Glutamine, 400 ug/ml hygromycin
Cryopreservation: 15% DMSO in FBS
Storage: Liquid nitrogen
Shipped: Dry ice

Provider
From the laboratory of Tom Misteli, PhD, National Cancer Institute/NIH.
References
  1. Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T, Danuser G, Misteli T. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol. 2007 Jun;9(6):675-82. Epub 2007 May 7.

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