Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Antibody Expression Plasmids

Plasmids expressing heavy and kappa chains, which when co-transfected into mammalian cells (such as 293F) will produce secreted antibody mBG86 that recognizes SARS-CoV-2 nucleocapsid antigen.

Highlights:

  • Recognizes SARS-CoV2 Nucleocapsid protein
  • mBG86 showed no cross reaction with SARS-CoV nucleocapsid or any of the other coronavirus N proteins tested by ELISA, indicating that mBG86 is very specific for SARS-CoV-2 nucleocapsid protein.
  • Suitable for ELISA, immunofluorescence assay, and western blot applications

A panel of mouse monoclonal antibodies targeting the SARS-CoV-2 N protein were generated and characterized. Truncated recombinant N protein was expressed and purified, used the recombinant antigen to immunize mice and generated a panel of hybridomas, and tested the resulting clones for activity in western blots, ELISAs, and immunofluorescence assays with SARS-CoV-2 infected cells. Cross-reactivity of the antibodies against SARS-CoV, MERS-CoV, HuCoV-NL63, HuCoV-HKU1, HuCoV-OC43and HuCoV-229E N protein was tested.

 

From the laboratory of Brian J. Geiss, PhD, Colorado State University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ECS013
pBG718, Anti-[mBG86] Heavy Chain Expression Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
ECS014
pBG719, Anti-[mBG86] Kappa Chain Expression Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
Specifications

Product Type: Plasmid
Gene/insert name: pBG718, Anti-[mBG86] Heavy Chain Expression Plasmid
Organism: E.coli
Antibiotic Resistance: Kanomycin
Grow in E. coli at 37 C: Yes
Cloning Site 5': SacI
Cloning Site 3': NotI
Insert Size: 1461 bp (Heavy) 723 bp (Kappa)
Vector Backbone and Size: pCMV (3952 bp)
High or low copy: High
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Brian J. Geiss, PhD, Colorado State University.
References

1. James S. Terry, Loran BR. Anderson, Michael S. Scherman, Carley E. McAlister, Rushika Perera, Tony Schountz, Brian J. Geiss. Development of a SARS-CoV-2 nucleocapsid specific monoclonal antibody. Virology.  2020 Sep 3:2020;09.03.280370. View Article

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