Heparan Sulfate Mutant Mouse Lung Endothelial Cell Lines

Exostosin Ext1-deficeint (Ext1-/-) endothelial cell line was generated by transiently transduce the immortalized wildtype "mother" mouse lung endothelial cell line which harbors conditionally targeted Ext1 (Ext1f/f) followed by cell cloning.

Exostosin-1 is a protein that in humans is encoded by the EXT1 gene. This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of Multiple Exostoses.

From the laboratory of Lianchun Wang, MD*, University of Georgia.

*Now affiliated with Georgia Institute of Technology.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EGA314
Mouse Lung Endothelial (Ext1f/f) Control Cell Line
1 Vial 4-6 weeks
Regular Price:$499.00
On Sale:
EGA315
Mouse Lung Endothelial (Ext1-/-) Cell Line
1 Vial 4-6 weeks
Regular Price:$499.00
On Sale:
Specifications

Product Type: Cell Line
Name: MLEC-Ext1 null or MLEC-Ext1 Control
Cell Type: Mouse lung endothelial cells
Morphology: Cobble stone-like
Organism: Mouse
Source: Lung
Biosafety Level: BSL 1
Subculturing: 1:3 split, Doubling every 3-4 days
Growth Conditions: High-glucose DMEM supplemented with 20% FBS, 30 ug/ml endothelial cell growth supplement and 50 ug/ml heparin.
Cryopreservation: Complete medium + 10% DMSO
Storage: Liquid Nitrogen
Shipped: Dry Ice

Provider

From the laboratory of Lianchun Wang, MD*, University of Georgia.

*Now affiliated with University of South Florida.

References
  1. Qiu H, Shi S, Yue J, et al. A mutant-cell library for systematic analysis of heparan sulfate structure-function relationships. Nat Methods. 2018;15(11):889-899.
  2. Capicciotti CJ, Zong C, Sheikh MO, Sun T, Wells L, Boons GJ. Cell-Surface Glyco-Engineering by Exogenous Enzymatic Transfer Using a Bifunctional CMP-Neu5Ac Derivative. J Am Chem Soc. 2017;139(38):13342-13348.
  3. Guo C, Fan X, Qiu H, Xiao W, Wang L, Xu B. High-resolution probing heparan sulfate-antithrombin interaction on a single endothelial cell surface: single-molecule AFM studies. Phys Chem Chem Phys. 2015;17(20):13301-13306.
  4. Qiu H, Jiang JL, Liu M, Huang X, Ding SJ, Wang L. Quantitative phosphoproteomics analysis reveals broad regulatory role of heparan sulfate on endothelial signaling. Mol Cell Proteomics. 2013;12(8):2160-2173.

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