EBV-negative Burkitt lymphoma cells, from a 5-years old African girl, transduced with KSHV latent protein LANA using, or as untransduced control.
Burkitt lymphoma is a rare but highly aggressive (fast-growing) B-cell non-Hodgkin lymphoma (NHL). This disease may affect the jaw, central nervous system, bowel, kidneys, ovaries, or other organs. Burkitt lymphoma may spread to the central nervous system (CNS).
From the laboratory of Subhash C. Verma PhD, University of Nevada, Reno.
|Product Type:||Cell Line|
|Cell Type:||Burkitt Lymphoma (B-Cells)|
|Source:||Donor: EBV-negative y-years old African girl with Burkitt lymphoma|
|Morphology:||Round to polymorphic cells that grow in suspension as single cells and in clumps|
Culture can be maintained by addition of fresh medium every 2-3 days, depending on the cell density. Maintain cell density between 5 to 7 x 10
|Growth Conditions:||RPMI 1640 medium (Corning, MT10040CM), 90%; fetal bovine serum (Hyclone, SH30088.03), 10%; L-glutamine, 2mM; Penicllin-streptomycin, 5U/mL and 5g/mL; puromycin (Sigma, P8833), 2 μg/mL|
|Cryopreservation:||RPMI 1640, 40%; Fetal bovine serum, 50%; DMSO, 10%.|
|Comments:||Standard procedure for handling frozen cells as recommended by ATCC should be followed. Seeding density: 2 to 5 x 105 viable cells/mL|
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