Anti-RNA dependent RNA polymerase R2 Antibodies

These mouse IgG1 monoclonal antibodies were raised against recombinant GST-R2 fusion protein and recognize RNA dependent RNA polymerase R2 of SARS-coronavirus (CoV).

Highlights:

  • Reacts with RNA dependent RNA polymerase R2 of SARS-CoV
  • Suitable for Western Blot and Immunofluorscence applications

The replication of viral RNA genomes is unique considering that the host cell does not contain a RNA dependent RNA polymerase. To overcome this constraint, the majority of RNA viruses encode their own RNA polymerase that is either packaged with the virus genome or is synthesized shortly after infection. This particular RNA dependent RNA polymerase, named as R2, is encoded by the SARS coronavirus. Severe acute respiratory syndrome (SARS) is a viral respiratory disease of zoonotic origin caused by the SARS-coronavirus (SARS-CoV). Coronaviruses are a major cause of upper respiratory diseases in humans.

From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.

Catalog Number Product Size AVAILABILITY Price Qty
ESG004
Anti-RNA dependent RNA polymerase R2 [4E6] Antibody, 5mL (supernatant)
5mL supernatant 3-5 weeks
Regular Price:$250.00
ESG005
Anti-RNA dependent RNA polymerase R2 [3H2] Antibody, 5mL (supernatant)
5mL supernatant 3-5 weeks
Regular Price:$250.00
ESG006
Anti-RNA dependent RNA polymerase R2 [3G2] Antibody, 5mL (supernatant)
5mL supernatant 3-5 weeks
Regular Price:$250.00
Specifications

Product Type: Antibody
Antigen: RNA dependent RNA polymerase R2 of SARS-coronavirus
Isotype: IgG1
Clonality: Monoclonal
Clone Name: 6 E6
3H2
3G2
Reactivity: SARS-CoV
Immunogen: Recombinant GST-R2 fusion protein
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB
Storage: 80C
Shipped: Dry Ice

Data

Western Blot

Western blot analysis of ascites using Hela cell lysate (transfected).

References
  1. Meng B, Lui YW, Meng S, Cao C, Hu Y. Identification of Effective siRNA Blocking the Expression of SARS Viral Envelope E and RDRP Genes. Mol Biotechnol. 2006 Jun; 33(2):141 - 8.
  2. Cheng A, Zhang W, Xie Y, Jiang W, Arnold E, Sarafianos SG, Ding J. Expression, purification, and haracterization of SARS coronavirus RNA polymerase. Virology. 2005 May; 335(2):165 -176.
  3. Sugiyama T, Cam H, Verdel A, Moazed D, Grewal SI. RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production. Proc Natl Acad Sci U S A 2005 Jan; 102(1):152-7.

If you publish research with this product, please let us know so we can cite your paper.

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