CN-alkyne is a linear C-nucleophile based chemoselective probe for detecting sulfenylated proteins.
Mild oxidation can convert the sulfhydryl group of cysteine residues on proteins to cysteine-sulfenic acid derivatives (Cys-SOH). Protein sulfenylation is a post-translational modification that is relevant to redox signaling. CN-alkyne shows more than 150-fold enhanced reactivity (compared to dimedone) towards a peptide based small molecule sulfenic acid model.
From the laboratory of Kate Carroll, PhD, The Scripps Research Institute.
|Product Type:||Small Molecule|
|Solubility:||Stock solution in DMSO (up to 500 mM), then dilute to appropriate concentration in aqueous buffer with final DMSO concentration <5%|
|Stability:||>6 months at -30C|
|Spectral Information:||1H-NMR (400 MHz, CDCl3): δ 7.59 ? 7.63 (m, 1H), 7.53 ? 7.58 (t, 1H, J = 8.0 Hz), 7.50 ? 7.52 (m, 1H), 7.27 ? 7.31 (m, 1H), 4.18 (t, 2H, J = 6.1 Hz); 4.07 (s, 2H), 2.44 (dt, 2H, J 1 = 7.0 Hz, J 2 = 2.6 Hz), 2.05 (p, 2H, J = 6.2 Hz), 2.01 (t, 1H, J = 2.7 Hz); 13C-NMR (in CDCl3, 100 Mhz): δ 159.9, 137.9, 131.1, 122.6, 121.1, 113.8, 110.6, 83.1, 69.5, 67.1, 45.9, 28.0, 15.3. LR-MS, m/z for C13H13NO3S; Calculated: 263.31; Observed: 264.1 (M++1).|
|Storage:||Room temperature for short-term, -30C for long term storage.|
If you publish research with this product, please let us know so we can cite your paper.