Recombinant Microbial Glutamate Dehydrogenase (GDH)

Glutamate Dehydrogenase (GDH) produced recombinantly in E. coli.


  • Microbial origin - Free from any animal or plant related contamination
  • Recombinant source - Fast and sustainable production in a non-pathogenic strain of E. coli
  • Extremely reliable- Maintains high activity in a wide range of temperatures (Close to optimal activity between 30C and 70C)
  • High thermal stability- Maintains over 85% of its activity for 8 hours at 50C

GDH is an oxidoreductase enzyme which relates carbon and nitrogen metabolism. It catalyzes the reduction of α-ketoglutarate and ammonia to L-glutamate and vice versa. This enzyme is a robust and ideal candidate for research use, and industrial applications in the diagnostics and food industries.

Catalog Number Product Size AVAILABILITY Price Qty
Recombinant Microbial Glutamate Dehydrogenase (GDH), 100mg
enz_gdh_003 , 100mg In stock
Regular Price:$47.00
Recombinant Microbial Glutamate Dehydrogenase (GDH), 500mg
enz_gdh_003 , 500mg In stock
Regular Price:$225.00
Recombinant Microbial Glutamate Dehydrogenase (GDH), 1g
enz_gdh_003 , 1g In stock
Regular Price:$440.00

Please contact us for more information about bulk quantities of this product.


Product Type: Protein
Name: Glutamate dehydrogenase (GDH)
CAS number: 9001-46-1
EC Number:
Source: E. coli(recombinant enzyme from a thermophilic bacterium)
Molecular Weight: 270 kDa; Homohexameric ( 45 kDa per subunit)
Format: Lyophilized powder
Buffer: 0.05 M Tris base and 0.5 M NaCl (before lyophilizing)
Stability: At -20ºC, it keeps 100% of its activity after one year
Concentration: > 13% (w/w)
Activity: > 90 U/mg protein; One unit is defined as the conversion of 1?mol of ?-ketoglutarate into glutamate, in 1 minute at 50°C at pH 8.0
Temperature Range: 20-70ºC (optimal: 50ºC)
pH Range: 7-8.5 (optimal: 8)
Storage: At -20 ºC
Shipped: Cold packs


Research and Diagnostics:

  • Determining levels of urea and ammonia in body fluids (e.g. plasma)
  • Control and standard in testing/assay validation
  • Positive control in glutamate dehydrogenase detection kits (e.g., for diagnosis of hospital-born infection by Clostridium difficile)


  • Aroma and flavor development in cheese
  • pre-alcoholic fermentation analysis in wine production (e.g., ammonia determination)
  • detecting spoilage in meat, fish, seafood, cheese, juices and milk products
  • As part of the synthesis process of L-amino acids (e.g. L-glutamine)

Glutamate dehydrogenase activity at different temperatures

Swissaustral GDH is more stable than GDH from bovine liver sources in a broad spetrum of temperatures offering close tooptimal activity between 30C and 70C.

  1. D P. Hornby, M J. Aitchison, P C.Engel. (1984).The kinetic mechanism of ox liver glutamate dehydrogenase in the presence of the allosteric effector ADP. The oxidative deamination of L-glutamate. Biochemical Journal 1984-10-01.
  2. M. Amenábar, J. Blamey. (2011). Purification and characterization of a thermostable glutamate dehydrogenase from a thermophilic bacterium isolated from a sterilization drying oven. Biochemistry and Molecular Biology Reports. 2, 91-95.
  3. J. DiRuggiero, F. Robb, R. Jagus, H. Klump, K. Borges, M. Kessel, X. Mai, M. Adams. (1993). Characterization, cloning, and in Vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaeon, ES4. Journal of Biological Chemistry. 268,17767-17774.

If you publish research with this product, please let us know so we can cite your paper.