Recombinant Microbial Glutamate Dehydrogenase (GDH)

Glutamate Dehydrogenase (GDH) produced recombinantly in E. coli.


  • Microbial origin - Free from any animal or plant related contamination
  • Recombinant source - Fast and sustainable production in a non-pathogenic strain of E. coli
  • Extremely reliable- Maintains high activity in a wide range of temperatures (Close to optimal activity between 30C and 70C)
  • High thermal stability- Maintains over 85% of its activity for 8 hours at 50C

GDH is an oxidoreductase enzyme which relates carbon and nitrogen metabolism. It catalyzes the reduction of α-ketoglutarate and ammonia to L-glutamate and vice versa. This enzyme is a robust and ideal candidate for research use, and industrial applications in the diagnostics and food industries.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Recombinant Microbial Glutamate Dehydrogenase (GDH), 100mg
enz_gdh_003 , 100mg In stock
Regular Price:$50.00
On Sale:
Recombinant Microbial Glutamate Dehydrogenase (GDH), 500mg
enz_gdh_003 , 500mg In stock
Regular Price:$240.00
On Sale:
Recombinant Microbial Glutamate Dehydrogenase (GDH), 1g
enz_gdh_003 , 1g In stock
Regular Price:$465.00
On Sale:

Please contact us for more information about bulk quantities of this product.


Product Type: Protein
Name: Glutamate dehydrogenase (GDH)
CAS number: 9001-46-1
EC Number:
Source: E. coli(recombinant enzyme from a thermophilic bacterium)
Molecular Weight: 270 kDa; Homohexameric ( 45 kDa per subunit)
Format: Lyophilized powder
Buffer: 0.05 M Tris base and 0.5 M NaCl (before lyophilizing)
Stability: At -20ºC, it keeps 100% of its activity after one year
Concentration: > 13% (w/w)
Activity: > 90 U/mg protein; One unit is defined as the conversion of 1?mol of ?-ketoglutarate into glutamate, in 1 minute at 50°C at pH 8.0
Temperature Range: 20-70ºC (optimal: 50ºC)
pH Range: 7-8.5 (optimal: 8)
Storage: At -20 ºC
Shipped: Cold packs


Research and Diagnostics:

  • Determining levels of urea and ammonia in body fluids (e.g. plasma)
  • Control and standard in testing/assay validation
  • Positive control in glutamate dehydrogenase detection kits (e.g., for diagnosis of hospital-born infection by Clostridium difficile)


  • Aroma and flavor development in cheese
  • pre-alcoholic fermentation analysis in wine production (e.g., ammonia determination)
  • detecting spoilage in meat, fish, seafood, cheese, juices and milk products
  • As part of the synthesis process of L-amino acids (e.g. L-glutamine)

Glutamate dehydrogenase activity at different temperatures

Swissaustral GDH is more stable than GDH from bovine liver sources in a broad spetrum of temperatures offering close tooptimal activity between 30C and 70C.

  1. D P. Hornby, M J. Aitchison, P C.Engel. (1984).The kinetic mechanism of ox liver glutamate dehydrogenase in the presence of the allosteric effector ADP. The oxidative deamination of L-glutamate. Biochemical Journal 1984-10-01.
  2. M. Amenábar, J. Blamey. (2011). Purification and characterization of a thermostable glutamate dehydrogenase from a thermophilic bacterium isolated from a sterilization drying oven. Biochemistry and Molecular Biology Reports. 2, 91-95.
  3. J. DiRuggiero, F. Robb, R. Jagus, H. Klump, K. Borges, M. Kessel, X. Mai, M. Adams. (1993). Characterization, cloning, and in Vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaeon, ES4. Journal of Biological Chemistry. 268,17767-17774.
  4. Wong SJ, Ringel AE, Yuan W, Paulo JA, Yoon H, Currie MA, Haigis MC. Development of a colorimetric α-ketoglutarate detection assay for prolyl hydroxylase domain (PHD) proteins. J Biol Chem. 2021 Feb 8:100397. View article 

If you publish research with this product, please let us know so we can cite your paper.