These CH12F3 B lymphocyte cell lines are useful tools to study the role of DNA metabolism in modulating immunoglobulin (Ig) heavy chain class switch recombination (CSR).
CH12F3 is capable of cytokine-induced Ig heavy chain CSR. Key enzymes involved in DNA recombination and joining double strand breaks (DSBs), such as DNA Ligase 1, Ligase 3, Ligase 4 and Apyrimidinic Endonuclease APE1, have been knocked out in these CH12F3 cell lines, providing ideal genetic materials to investigate the roles of these proteins in regulating the proficient CSR event in mature B lymphocyte cell lines. Since enzymes involved in DNA metabolism play essential roles in embryogenesis, knockout of these proteins often resulted in lethality in mouse embryonic stem cells. However, knockout of these proteins in mature B cells are compatible with cell viability.
From the laboratory of Kefei Yu, PhD, Michigan State University.
|Product Type:||Cell Line|
|Name:||CH12F3 (APE1-/-/-APE2-) Cell Line|
|Cell Type:||B lymphocyte|
|Accession ID:||P27695, Q59KZ1|
|Growth Conditions:||RPMI1640 supplemented with 10% FBS|
|Subculturing:||Grow in suspension; 1:5 split every 2-3 days|
|Cryopreservation:||Growth medium plus 10% DMSO|
If you publish research with this product, please let us know so we can cite your paper.