Anti-Zaire Ebola Virus VP35 Protein, C-Terminal [6C5] Antibody
This mouse IgG2a monoclonal antibody [6C5] was generated against DNA immunization followed by recombinant protein and is specific for the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein.
Highlights:
- Reacts with the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein
- Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 6C5
Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.
From a laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai.
This mouse IgG2a monoclonal antibody [6C5] was generated against DNA immunization followed by recombinant protein and is specific for the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein.
Highlights:
- Reacts with the C-terminal portion of Zaire ebolavirus polymerase cofacter VP35 protein
- Suitable for Western Blot, Immunoprecipitation and Immunofluorescence applications
Recombinant versions available from our sister company, Absolute Antibody:
- Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e., specificity) from the hybridoma 6C5
Ebola virus (EBOV), or sometimes referred to as Zaire ebolavirus is one of the five identified Ebola virus species. Zaire ebolavirus is a virological taxon that comes from the genus, Ebolvarius, family Filoviridae and the order Mononegavirales. Ebola, often fatal has been shown to cause hemorrhagic fever in humans and other mammals including monkeys and chimpanzees. Polymerase cofactor VP35 acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. It prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3). This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1. VP35 also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.
From a laboratory of Christopher F. Basler, PhD, Icahn School of Medicine at Mount Sinai.
| Catalog Number | Product | DataSheet | Size | AVAILABILITY | Price | Qty |
|---|
| Product Type: | Antibody |
| Accession ID: | AF086833.2 Ebola Mayinga, Zaire 1976 |
| Antigen: | Zaire ebolavirus polymerase cofactor VP35 |
| Molecular Weight: | 35 kDa |
| Isotype: |
EMS702: IgG2a Recombinant versions: see product name |
| Clonality: | Monoclonal |
| Clone Name: | 6C5 |
| Reactivity: | Virus |
| Immunogen: | DNA immunization followed by recombinant protein |
| Species Immunized: | Mouse |
| Epitope: | Within C-terminal portion of protein |
| Purification Method: | Protein G Affinity |
| Buffer: |
EMS702: PBS with 0.05% (w/v) Sodium Azide Recombinant versions: PBS with 0.02% Proclin 300 |
| Tested Applications: | WB (1:1000), IP (1:500), IF |
| Storage: | -20C |
| Shipped: | Cold packs |
- Olsen ME, Filone CM, Rozelle D, Mire CE, Agans KN, Hensley L, Connor JH.Polyamines and Hypusination Are Required for Ebolavirus Gene Expression and Replication. MBio. 2016 Jul 26;7(4). pii: e00882-16. View Article
- Takamatsu Y, Kolesnikova L, Becker S. Ebola virus proteins NP, VP35, and VP24 are essential and sufficient to mediate nucleocapsid transport. Proc Natl Acad Sci U S A. 2018 Jan 16. pii: 201712263. View Article
- Fang J, Pietzsch C, Ramanathan P, Santos RI, Ilinykh PA, Garcia-Blanco MA, Bukreyev A, Bradrick SS. Staufen1 Interacts with Multiple Components of the Ebola Virus Ribonucleoprotein and Enhances Viral RNA Synthesis. MBio. 2018 Oct 9;9(5). pii: e01771-18. View Article
- Miyake T, Farley CM, Neubauer BE, Beddow TP, Hoenen T, Engel DA. Ebola Virus Inclusion Body Formation and RNA Synthesis Are Controlled by a Novel Domain of Nucleoprotein Interacting with VP35. J Virol. 2020;94(16):e02100-19. Published 2020 Jul 30. View Article
- Takamatsu Y, Kolesnikova L, Schauflinger M, Noda T, Becker S. The Integrity of the YxxL Motif of Ebola Virus VP24 Is Important for the Transport of Nucleocapsid-Like Structures and for the Regulation of Viral RNA Synthesis. J Virol. 2020 Apr 16;94(9):e02170-19. View Article
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