Branched Chain Amino-acid Transaminase-2 (BCAT2); Full-length Human

Branched chain aminotransferase (BCAT2; EC 2.6.1.42) is an aminotransferase enzyme which acts upon branched-chain amino acids. It uses largely α-ketoglutarate in forming branched chain α-keto acids and glutamate. The biological function of branched chain aminotransferases is to catalyse the synthesis of the branched chain amino acids leucine, isoleucine, and valine.

 

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Catalog Number Product Size AVAILABILITY Price Qty
FCA010
Branched Chain Amino-acid Transaminase-2 (BCAT2); Full-length Human
25ug (1mg/mL) In stock
Price: $209.00
Specifications
Product Type: Protein
Source: E. Coli
Molecular Weight: 44.03 kDa
Format: Liquid
Purity: 95% (densitometry)
Buffer: 50 mM potassium phosphate pH-7.4, 50 mM sodium chloride, 0.5 mM ethylenediaminetetraaceticacid, and 2.5% glycerol.
Concentration: 1.0mg/mL
Comments: Human branch-chained aminotransferase-2 , (Accession No. BC004243), aa 390 (full length minus the first 16 aa) with N-terminal hexahistidine tag, MW= 44.01 kDa, expressed in E.coli.
Storage: Stable > 6 months at -80C
Shipped: Dry ice
Comments

SDS-Page:

Specific Activity: 10.7 pmol/min/ug.  One unit is defined as the amount of enzyme that will convert 1 nmol of  NADP to NADPH at 30C.  Assay conditions: 100 mM sodium phosphate, pH 7.4,  5 mM DTT, 50mM ammonium sulfate, 5 µM pyroxidal 5’ phosphate, 0.5 mM 3-acetyl pyridine adenine dinucleotide, 0.5 mM a-ketoglutarate, 5 mM leucine, 12.5 mM EDTA, 10 units/ml leucine dehydrogenase, and 10 nM human branch-chained aminotransferase-2  at  30C for 30 min.

 

 

Enzyme activation: Maximal activity of human branch-chained aminotransferase-2 requires pretreatment of enzyme with 100 mM final DTT for at least 1 hour at 4oC.

References

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