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Specifications
| Product Type: | Antibody |
| Antigen: | ABCD2 peptide |
| Accession ID: | Gene ID: 26874 |
| Molecular Weight: | 78 kDa |
| Clonality: | Polyclonal |
| Reactivity: | Mouse, Rat and Human |
| Immunogen: | Fusion Peptide |
| Species Immunized: | Rabbit |
| Epitope: | Aa377-711 |
| Buffer: | 50% glycerol, 50mM tris (pH 7.4) and 0.01% azide |
| Tested Applications: | WB (1:2000), IF (1:200), IEM (1:50) |
| Storage: | -80C |
| Shipped: | Dry Ice |
Data
Immunofluorescence Microscopy and Immunoblot Analysis of D2 Protein in Cultured Cells.
(A) Cells were transfected with a plasmid containing a cDNA encoding mouse D2. Cells were processed for indirect immunofluorescence microscopy using the polyclonal D2 antibody. Inset: lysates from cells transfected with control plasmid (V) and D2 were analyzed by SDS-PAGE and immunoblotting. (B) Total membranes were prepared from adipose collected from wild-type and D2-deficient (D2-/-) mice and analyzed by immunoblotting with the D2 antibody.
Provider
From the laboratory of Greg A. Graf, PhD, University of Kentucky.
References
- Liu J, Sabeva NS, Bhatnagar S, Li XA, Pujol A, Graf GA. ABCD2 is abundant in adipose tissue and opposes the accumulation of dietary erucic acid (C22:1) in fat. J Lipid Res. 2010 Jan;51(1):162-8.
- Liu J, Liang S, Liu X, Brown JA, Newman KE, Sunkara M, Morris AJ, Bhatnagar S, Li X, Pujol A, Graf GA. The absence of ABCD2 sensitizes mice to disruptions in lipid metabolism by dietary erucic acid. J Lipid Res. 2012 Jun;53(6):1071-9.
- Liu X, Liu J, Lester JD, Pijut SS, Graf GA. ABCD2 identifies a subclass of peroxisomes in mouse adipose tissue. Biochem Biophys Res Commun. 2015 Jan 2;456(1):129-34.
If you publish research with this product, please let us know so we can cite your paper.