Anti-Zebrafish Mind Bomb (mib) [6B2/H4] Antibody

This mouse IgG2b monoclonal antibody was raised against recombinant GST-fusion-fish mib protein and is specific for Zebrafish mind bomb (mib).

Highlights:

  • Reacts with Zebrafish mind bomb (mib)
  • Suitable for Western Blot and Immunofluorscence applications

Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells (Itoh et al., 2003). In addition, there are a series of mib alleles with different genetic severity (Zhang et al., 2007a). The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein (Zhang et al., 2007a, 2007b).

From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.

Catalog Number Product Size AVAILABILITY Price Qty
ESG003
Anti-Zebrafish Mind Bomb (mib) [6B2/H4] Antibody, 5mL (supernatant)
5mL supernatant 3-5 weeks
Price: $250.00
Specifications
Product Type: Antibody
Antigen: Zebrafish mind bomb (mib)
Isotype: IgG2b
Clonality: Monoclonal
Clone Name: 6B2/H4
Reactivity: Zebrafish
Immunogen: Recombinant GST fusion-fish mib protein
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IF
Storage: 80C
Shipped: Dry Ice
Data

Immunofluorescence

Immunofluorescence of COS7 cells transfected with FLAG-Mib, stained with hybridoma supernatant (A) 6B2 and (B) H4 and positive control, a-FLAG. 6B2 epitope and H4 epitope were detected with Alexa 488 (green), and FLAG epitope with Alexa 568 (red).

References
  1. MA, Kellam P, Drosten C. Human infection with MERS coronavirus after exposure to infected camels, Saudi Arabia, 2013. Emerg Infect Dis. 2014 Jun;20(6):1012-5.
  2. de Groot RJ, Baker SC, Baric RS, Brown CS, Drosten C, Enjuanes L, Fouchier RA,Galiano M, Gorbalenya AE, Memish ZA, Perlman S, Poon LL, Snijder EJ, Stephens GM,Woo PC, Zaki AM, Zambon M, Ziebuhr J. Middle East respiratory syndrome coronavirus (MERSCoV): announcement of the Coronavirus Study Group. J Virol. 2013 Jul;87(14):7790-2.
  3. Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012 Nov 8;367(19):1814-20.
  4. If you publish research with this product, please let us know so we can cite your paper.

 
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