Anti-Zebrafish Mind Bomb (mib) [6B2/H4] Antibody
![Anti-Zebrafish Mind Bomb (mib) [6B2/H4] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1742_2001202001265917410.jpg "Anti-Zebrafish Mind Bomb (mib) [6B2/H4] Antibody")
This mouse IgG2b monoclonal antibody was raised against recombinant GST-fusion-fish mib protein and is specific for Zebrafish mind bomb (mib).
Highlights:
- Reacts with Zebrafish mind bomb (mib)
- Suitable for Western Blot and Immunofluorscence applications
Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells (Itoh et al., 2003). In addition, there are a series of mib alleles with different genetic severity (Zhang et al., 2007a). The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein (Zhang et al., 2007a, 2007b).
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
This mouse IgG2b monoclonal antibody was raised against recombinant GST-fusion-fish mib protein and is specific for Zebrafish mind bomb (mib).
Highlights:
- Reacts with Zebrafish mind bomb (mib)
- Suitable for Western Blot and Immunofluorscence applications
Zebrafish mind bomb (mib) was isolated in a large-scale screen aiming for morphologically discernible phenotypes. Mib mutants have phenotypes similar to Notch pathway mutants: a dramatic increase of primary neurons and somatic segmentation abnormalities. mib was positional cloned to be a novel gene. Mib is a novel E3 ligase with three Ring Finger (RF) domains in the C-terminal. Mib can interact with Delta, a Notch ligand. The interaction will mediate a RF-dependent ubiquitination and endocytosis of Delta, and thus effectively activates Notch in neighbouring cells (Itoh et al., 2003). In addition, there are a series of mib alleles with different genetic severity (Zhang et al., 2007a). The most severe phenotype of antimorphic mibta52b allele is partly due to the antagonization of a second Mib homolog, Mib2, by Mibta52b protein (Zhang et al., 2007a, 2007b).
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
| Product Type: | Antibody |
| Antigen: | Zebrafish mind bomb (mib) |
| Accession ID: | Q86YT6 |
| Isotype: | IgG2b |
| Clonality: | Monoclonal |
| Clone Name: | 6B2/H4 |
| Reactivity: | Zebrafish |
| Immunogen: | Recombinant GST fusion-fish mib protein |
| Species Immunized: | Mouse |
| Buffer: | Cell culture supernatant |
| Tested Applications: | WB, IF |
| Storage: | 80C |
| Shipped: | Cold Packs |
Immunofluorescence
Immunofluorescence of COS7 cells transfected with FLAG-Mib, stained with hybridoma supernatant (A) 6B2 and (B) H4 and positive control, a-FLAG. 6B2 epitope and H4 epitope were detected with Alexa 488 (green), and FLAG epitope with Alexa 568 (red).
From the laboratory of A*STAR Institute of Molecular and Cell Biology (IMCB) Monoclonal Antibody Unit.
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