RealThiol AM Ester (RT-AM) Glutathione (GSH) Probe
RealThiol AM ester (RT-AM) provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
Highlights:
- RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage
- Easily quantitate GSH levels in live cells using confocal microscopy/FACS
- Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve
- For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
RealThiol AM ester (RT-AM) provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
Highlights:
- RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage
- Easily quantitate GSH levels in live cells using confocal microscopy/FACS
- Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve
- For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
Specifications
| Product Type: | Small Molecule |
| Name: | RealThiol AM ester (RT-AM) |
| Chemical Formula: | C26H25N3O11 |
| Molecular Weight: | 555.16 |
| Format: | Red to orange solid |
| Purity: | >97%, HPLC |
| Tested Applications: | 1 µM for cell staining |
| Solubility: | DMSO |
| Spectral Information: | ex/em: RealThiol: 488/565; RealThiol-GSH: 405/488 |
| Platform: | Confocal microscopy/FACS |
| Compatible Cells: | Mammalian cells e.g. HeLa, HT1080 |
| Detection Method: | Fluorescence |
| Comments: |
- RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage. - Dilute DMSO stock with imaging buffer. - A laser-based light source is preferred for best results. |
| Storage: | -80C |
| Shipped: | Ambient Temperature |
Provider
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
References
- Jiang X, Chen J, Bajić A, Zhang C, Song X, Carroll SL, Cai ZL, Tang M, Xue M, Cheng N, Schaaf CP, Li F, MacKenzie KR, Ferreon ACM, Xia F, Wang MC, Maletić-Savatić M, Wang J. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.
- Oberstadt M, Stieler J, Simpong DL, Römuß U, Urban N, Schaefer M, Arendt T, Holzer M. TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole. Sci Rep. 2018 Feb 2;8(1):2248. View Article
- Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article
- Li W, Chen C, Drelich A, Martinez DR, Gralinski LE, Sun Z, Schäfer A, Kulkarni SS, Liu X, Leist SR, Zhelev DV, Zhang L, Kim YJ, Peterson EC, Conard A, Mellors JW, Tseng CK, Falzarano D, Baric RS, Dimitrov DS. Rapid identification of a human antibody with high prophylactic and therapeutic efficacy in three animal models of SARS-CoV-2 infection. Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29832-29838. View Article
If you publish research with this product, please let us know so we can cite your paper.
