RealThiol AM ester (RT-AM) provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
|Product Type:||Small Molecule|
|Name:||RealThiol AM ester (RT-AM)|
|Format:||Red to orange solid|
|Tested Applications:||1 µM for cell staining|
|Spectral Information:||ex/em: RealThiol: 488/565; RealThiol-GSH: 405/488|
|Compatible Cells:||Mammalian cells e.g. HeLa, HT1080|
- RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage.
- Dilute DMSO stock with imaging buffer.
- A laser-based light source is preferred for best results.
If you publish research with this product, please let us know so we can cite your paper.