RealThiol AM Ester (RT-AM) Glutathione (GSH) Probe

RealThiol AM ester (RT-AM) provides ratiometric fluorescent readout for quantification of GSH in live mammalian cells.


  • RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage
  • Easily quantitate GSH levels in live cells using confocal microscopy/FACS
  • Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve
  • For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe

Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.

From the laboratory of Jin Wang, PhD, Baylor College of Medicine.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
RealThiol AM Ester (RT-AM) Glutathione (GSH) Probe
50nmol In stock
Regular Price:$218.00
On Sale:

Product Type: Small Molecule
Name: RealThiol AM ester (RT-AM)
Chemical Formula: C26H25N3O11
Molecular Weight: 555.16
Format: Red to orange solid
Purity: >97%, HPLC
Tested Applications: 1 µM for cell staining
Solubility: DMSO
Spectral Information: ex/em: RealThiol: 488/565; RealThiol-GSH: 405/488
Platform: Confocal microscopy/FACS
Compatible Cells: Mammalian cells e.g. HeLa, HT1080
Detection Method: Fluorescence
Comments: - RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage.
- Dilute DMSO stock with imaging buffer.
- A laser-based light source is preferred for best results.
Storage: -80C
Shipped: Ambient Temperature

From the laboratory of Jin Wang, PhD, Baylor College of Medicine.
  1. Jiang X, Chen J, Bajić A, Zhang C, Song X, Carroll SL, Cai ZL, Tang M, Xue M, Cheng N, Schaaf CP, Li F, MacKenzie KR, Ferreon ACM, Xia F, Wang MC, Maletić-Savatić M, Wang J. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.
  2. Oberstadt M, Stieler J, Simpong DL, Römuß U, Urban N, Schaefer M, Arendt T, Holzer M. TDP-43 self-interaction is modulated by redox-active compounds Auranofin, Chelerythrine and Riluzole. Sci Rep. 2018 Feb 2;8(1):2248. View Article
  3. Jiang X, Chen J, Wang MC, Wang J. Glutathione Quantification in Live Cells with Real-Time Imaging and Flow Cytometry. STAR Protoc. 2020 Nov 14;1(3):100170. View Article 
  4. Li W, Chen C, Drelich A, Martinez DR, Gralinski LE, Sun Z, Schäfer A, Kulkarni SS, Liu X, Leist SR, Zhelev DV, Zhang L, Kim YJ, Peterson EC, Conard A, Mellors JW, Tseng CK, Falzarano D, Baric RS, Dimitrov DS. Rapid identification of a human antibody with high prophylactic and therapeutic efficacy in three animal models of SARS-CoV-2 infection. Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29832-29838.  View Article 

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