Anti-BM180 (basement membrane, 180kDa) [3E12] Antibody

This IgG2a mouse monoclonal antibody [3E12] was generated against mouse BM180 (basement membrane 180 kDa) protein derived from EHS tumor cells and is specific for mouse BM180.

Highlights

  • Reacts with mouse BM180
  • Suitable for Western Blot, ELISA and Immunohistochemistry

BM180 is a novel basement membrane component with a role in regulated tear secretion by lacrimal acinar cells. BM180 bears some sequence similarity to alpha-gliadin, a plant protein against which antibodies have been reported in patients with Sjogren's syndrome. BM180 has an electrophoretic mobility of 180 and 60 kDa in the absence or presence of dithiothreitol, respectively, and shows no immunological identity by competitive enzyme-linked immunosorbent assay with laminin 1, collagen IV, entactin, fibronectin, BM-40, perlecan, or vitronectin.

From the laboratory of Gordon W. Laurie, PhD, Rebecca A. Ogle, PhD and J. Douglas Glass, MD, University of Virginia

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EG1801
Anti-BM180 (basement membrane, 180kDa) [3E12] Antibody, 100ug
100ug 2-3 weeks
Price: $323.00
Specifications
Product Type: Antibody
Antigen: BM180
Molecular Weight: 60 kDa (reduced)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 3 E12
Reactivity: Mouse
Immunogen: Mouse BM180 protein derived from EHS tumor cells
Species Immunized: Rat
Purification Method: Protein G Affinity Chromatography
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, ELISA and IHC
Storage: -20C
Shipped: Cold packs
Data

Western Blot

Presence of BM180 in peak 2. Lanes 1 and 2, negative control BMS blots with and without DTT (in which secondary antibody alone was applied to blot). Lanes 3 (without DTT, -) and 5 (with DTT, +), 3E12 MAb detection of BM180 in peak 2 prepared by solubilization of BMS with 30% ammonium sulfate. Lanes 4 (without DTT, -) and 6 (with DTT, +), detection of BM180 in peak 2 prepared by gel filtration.

Adapted from: Laurie GW., et al. Am J Physiol. 1996 Jun;270(6 Pt 1):C1743-50.

Provider
From the laboratory of Gordon W. Laurie, PhD, Rebecca A. Ogle, PhD and J. Douglas Glass, MD, University of Virginia
References
  1. Laurie GW, Glass JD, Ogle RA, Stone CM, Sluss JR, Chen L. "BM180": a novel basement membrane protein with a role in stimulus-secretion coupling by lacrimal acinar cells. Am J Physiol. 1996 Jun;270(6 Pt 1):C1743-50.

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