This IgG2a mouse monoclonal antibody [3E12] was generated against mouse BM180 (basement membrane 180 kDa) protein derived from EHS tumor cells and is specific for mouse BM180.
BM180 is a novel basement membrane component with a role in regulated tear secretion by lacrimal acinar cells. BM180 bears some sequence similarity to alpha-gliadin, a plant protein against which antibodies have been reported in patients with Sjogren's syndrome. BM180 has an electrophoretic mobility of 180 and 60 kDa in the absence or presence of dithiothreitol, respectively, and shows no immunological identity by competitive enzyme-linked immunosorbent assay with laminin 1, collagen IV, entactin, fibronectin, BM-40, perlecan, or vitronectin.
From the laboratory of Gordon W. Laurie, PhD, Rebecca A. Ogle, PhD and J. Douglas Glass, MD, University of Virginia
Part of The Investigator's Annexe program.
|Molecular Weight:||60 kDa (reduced)|
|Clone Name:||3 E12|
|Immunogen:||Mouse BM180 protein derived from EHS tumor cells|
|Purification Method:||Protein G Affinity Chromatography|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, ELISA and IHC|
Adapted from: Laurie GW., et al. Am J Physiol. 1996 Jun;270(6 Pt 1):C1743-50.
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