E. coli Strain ALS1074
Escherichia coli cells cannot metabolize xylose but can accumulate lactic acid from other carbohydrates such as glucose under anaerobic conditions.
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
Part of The Investigator's Annexe program.
Escherichia coli cells cannot metabolize xylose but can accumulate lactic acid from other carbohydrates such as glucose under anaerobic conditions.
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
Part of The Investigator's Annexe program.
Specifications
| Product Type: | Bacteria |
| Name: | ALS1074 |
| Cell Type: | Bacteria |
| Organism: | Escherichia coli |
| Strain: | ALS1074 |
| Genotype: | MG1655 ΔxylA748::(FRT) ΔpflB::Cam |
| Antibiotic Resistance: | Chloramphenicol |
| Growth Conditions: | Lysogeny broth: 10g/L tryptone, 10g/L NaCl, 5g/L yeast extract, pH adjusted to 7.0, aerobic 37C |
| Format: | Lyophilized culture |
| Storage: | Room temperature as lyophilized culture |
| Shipped: | Ambient temperature |
Documentation
Protocol Notes
- Inject 1 mL of sterile DI water into vial and gently mix.
- Use small portions of vial (liquid) contents to inoculate a sterile, liquid culture to an initial OD of 0.03-0.1. For this liquid culture, use the specified growth medium with or without an antibiotic as appropriate.
- When culture has visibly grown in liquid medium, plate on solid (Agar) growth medium and incubate at 37C. Maintain strain.
Research
| Strain | Phenotype |
| ALS974 | Accumulates lactic acid |
| ALS929 | Accumulates pyruvic acid |
| ALS1392 | Does not metabolize arabinose, glucose nor xylose |
| ALS1391 | Does not metabolize arabinose nor xylose |
| ALS1371 | Does not metabolize arabinose nor glucose |
| ALS1370 | Does not metabolize xylose nor glucose |
| ALS1074 | Does not metabolize xylose but can accumulate lactic acid from glucose |
| ALS1073 | Does metabolize glucose but can accumulate lactic acid from xylose |
| ALS1060 | Does not metabolize xylose nor glucose |
| ALS1059 | Accumulates pyruvic acid |
| ALS1058 | Does not metabolize glucose |
| ALS1048 | Does not metabolize glucose |
| ALS1038 | Does not metabolize xylose |
| ALS1054 | Accumulates pyruvic acid |
| ALS1122 | Does not metabolize xylose nor glucose |
Provider
From the laboratory of Mark A. Eiteman, PhD, University of Georgia.
References
- Eiteman MA, Lee SA, Altman R, Altman E. A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose. Biotechnol Bioeng. 2009 Feb 15;102(3):822-7.
- US Patent Number 8,551,758; PCT Publication number WO2008124162 A2. Pending applications in several jurisdictions University of Georgia Research Foundation, Inc.
If you publish research with this product, please let us know so we can cite your paper.
