This mouse IgG monoclonal antibody was generated against sieve elements isolated from California shield leaf tissue cultures and recognizes an antigen in the Arabidopsis ecotype Columbia that is associated specifically with the plasma membrane of sieve elements, but not companion cells.
Arabidopsis early nodulin (ENOD)-like proteins are encoded by a multigene family composed of several types of structurally related phytocyanins that have a similar overall domain structure of an amino-terminal signal peptide, plastocyanin-like copper-binding domain, proline/serine-rich domain, and carboxy-terminal hydrophobic domain. The amino- and carboxy-terminal domains of the 21.5-kD sieve element-specific ENOD are posttranslationally cleaved from the precursor protein, resulting in a mature peptide of approximately 15 kD that is attached to the sieve element plasma membrane via a carboxy-terminal glycosylphosphatidylinositol membrane anchor. Many of the Arabidopsis ENOD-like proteins accumulate in gametophytic tissues, whereas in both floral and vegetative tissues, the sieve element-specific ENOD is expressed only within the phloem.
From the laboratory of Richard D. Sjolund, PhD, University of Iowa.
Part of The Investigator's Annexe program.
|Antigen:||Early nodulin (ENOD)-like protein 9 (gene: At3g20570)|
|Molecular Weight:||15 kDa (mature peptide)|
|Immunogen:||Sieve elements isolated from California shield leaf (Streptanthus tortuosus; Brassicaceae) tissue cultures|
|Species Immunized:||BALB/c Mouse|
|Purification Method:||Cell culture supernatant|
|Buffer:||Cell culture supernatant with 0.05% Sodium azide|
|Tested Applications:||Immunolocalization, Immunohistochemistry|
|Storage:||4C short term, -80C long term (avoid multiple freeze-thaw cycles)|
Confocal microscopy immunolocalization of the SE-ENOD. A, Brightfield image showing the phloem (P) and xylem (X) in fresh tissue vibratome sections (50100 um) of cauliflower stem. B, The RS6 monoclonal antibody labeled only the phloem in the vascular bundles of cauliflower stem sections (bar = 200um).
Adapted from: Khan JA, et al. Plant Physiol. 2007 Apr;143(4):1576-89.
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