Microglial cell line (SIM-A9) expresses macrophage/microglia specific proteins, CD68 and Iba1.
Microglia cells share functional similarities with macrophages, and are thought to act as the primary immune cells of the central nervous system (CNS). They monitor their microenviornment and become activated upon detection of abnormal and/or offensive signals. Activated microglia have been found in inflammatory conditions such as brain/spinal cord injury, ischemic strokes, infections and neurodegenerative diseases. Recent findings indicate that microglia are also involved in learning and memory by regulating synaptic plasticity.
From the laboratory of Kumi Nagamoto-Combs, PhD, University of North Dakota.
|Product Type:||Cell Line|
|Cell Type:||Spontaneously immortalized microglia cell line|
|Morphology:||Heterogenous; spindle, multi-polar, spherical, flat|
|Source:||Postnatal day 1-2 mouse cerebral cortex|
|Growth Conditions:||DMEM/F12 with 5% DHS, 10% FBS, Pen-strep, neomycin (optional)|
|Cryopreservation:||10% DMSO in DMEM/F12 growth medium above|
Photomicrographs of SIM-A9 Cells
SIM-A9 cells expressing microglia/macrophage-specific proteins. SIM-A9 cells were treated without (Control; left panels) or with 2.5 ng/mL LPS(LPS; right panels) for 24 h prior to immunostaining for microglial markers, CD68 or Iba1, as well as for astrocyte and neuronal marker proteins, GFAP and MAP2, respectively. Scale bar = 50um.
Adapted from: Kumi Nagamoto-Combs., et al. J Neurosci Methods. 2014 Aug 15;233:187-98.
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