Anti-CREB-H (CREB3L3) Antibody

This rabbit IgG polyclonal antibody was generated against purified protein segment, N-terminal amino acids 75 to 250 of mouse cAMP-responsive element binding protein, hepatocyte specific (CREB-H) protein and reacts against human and mouse CREB-H.

Highlights

  • Exhibits high specificity and high titer
  • Reacts with human and mouse CREBH
  • Suitable for Western Blot, Immunoprecipitation and ChIP analysis applications

CREB-H (also called CREB3L3) is a stress sensor that plays key roles in lipid and glucose metabolism associated with metabolic disease. CREB-H is a liver-specific bZIP transcription factor with molecular weights of 52.7 kD (precursor predicted size) and 35.3 kD (activated form predicted size). The CREB-H protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation.

From the laboratory of Kezhong Zhang, PhD, Wayne State University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EWS101
Anti-CREB-H (CREB3L3) Antibody
100uL In stock
Regular Price:$376.00
Specifications

Product Type: Antibody
Accession ID: AAH28820.1
Antigen: CREB-H, CREB3L3
Molecular Weight: 52.7 kDa (predicted)
Isotype: IgG
Clonality: Polyclonal
Reactivity: Human and mouse
Immunogen: Purified protein segment, N-terminal 176 amino acids
Species Immunized: Rabbit
Epitope: AA 75 - 250 of CREB-H protein
Purification Method: Affinity Purified
Buffer: PBS/0.05% Azide, 50% Glycerol
Tested Applications: WB (1:1000-2000), IP-Western (1:1000), ChIP (1:1000)
Storage: -20C
Shipped: Cold packs

Documentation

Notes

For Western blot analysis with total raw lysates from liver tissues, the antibody can detect an unspecific signal around 90 kD when films are incubated with PDVF membranes for a relatively long time. But the unspecific signal can be easily separated or distinguished from the specific signals of CREB-H precursor (around 75 kD gel size) and activated (around 50 kD gel size) forms.

Data

IP-Western Blot Analysis

WB Data

IP-Western blot analysis of CREB-H and PPAR? interaction in the livers of mice under normal chow, after 14-hour fasting, or after 6 months of AHF diet. Whole-protein lysates were prepared from mouse liver tissues and subjected to IP to pull down the endogenous PPAR? or CREB-H protein complex using an anti-PPAR? or anti-CREB-H antibody. The precipitates were analyzed by immunoblotting with the antibody against CREB-H or PPAR?. Whole-liver protein lysates were subjected to Western blot analysis to determine the levels of CREB-H, PPAR?, and ?-actin as the controls. The values below the gels represent the interactive CREB-H (with PPAR?) and PPAR? (with CREB-H) protein signal intensities after normalization to ?-actin signal intensities. Ab, antibody; Ad, adenovirus.

Adapted from: Kim, H., et al. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82.

Provider
From the laboratory of Kezhong Zhang, PhD, Wayne State University.
References
  1. Kim, H., Mendez, R., Zheng, Z., Chang, L., Cai, J., Zhang, R., and Zhang, K. 2014. Liver-enriched Transcription Factor CREBH Interacts with Peroxisome Proliferator-activated Receptor ? to Regulate Metabolic Hormone FGF21. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82
  2. Zhang, C., Wang, G., Zheng, Z., Maddipati, K.R., Zhang, X., Dyson, G., Williams, P., Duncan, S.A., Kaufman, R.J., and Zhang, K. 2012. ER-tethered Transcription Factor CREBH Regulates Hepatic Lipogenesis, Fatty Acid Oxidation, and Lipolysis upon Metabolic Stress. Hepatology 55 (4): 1070-1082. PMCID: PMC3319338.

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