This rabbit IgG polyclonal antibody was generated against purified protein segment, N-terminal amino acids 75 to 250 of mouse cAMP-responsive element binding protein, hepatocyte specific (CREB-H) protein and reacts against human and mouse CREB-H.
CREB-H (also called CREB3L3) is a stress sensor that plays key roles in lipid and glucose metabolism associated with metabolic disease. CREB-H is a liver-specific bZIP transcription factor with molecular weights of 52.7 kD (precursor predicted size) and 35.3 kD (activated form predicted size). The CREB-H protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation.
From the laboratory of Kezhong Zhang, PhD, Wayne State University.
Part of The Investigator's Annexe program.
|Molecular Weight:||52.7 kDa (predicted)|
|Reactivity:||Human and mouse|
|Immunogen:||Purified protein segment, N-terminal 176 amino acids|
|Epitope:||AA 75 - 250 of CREB-H protein|
|Purification Method:||Affinity Purified|
|Buffer:||PBS/0.05% Azide, 50% Glycerol|
|Tested Applications:||WB (1:1000-2000), IP-Western (1:1000), ChIP (1:1000)|
For Western blot analysis with total raw lysates from liver tissues, the antibody can detect an unspecific signal around 90 kD when films are incubated with PDVF membranes for a relatively long time. But the unspecific signal can be easily separated or distinguished from the specific signals of CREB-H precursor (around 75 kD gel size) and activated (around 50 kD gel size) forms.
IP-Western Blot Analysis
IP-Western blot analysis of CREB-H and PPAR? interaction in the livers of mice under normal chow, after 14-hour fasting, or after 6 months of AHF diet. Whole-protein lysates were prepared from mouse liver tissues and subjected to IP to pull down the endogenous PPAR? or CREB-H protein complex using an anti-PPAR? or anti-CREB-H antibody. The precipitates were analyzed by immunoblotting with the antibody against CREB-H or PPAR?. Whole-liver protein lysates were subjected to Western blot analysis to determine the levels of CREB-H, PPAR?, and ?-actin as the controls. The values below the gels represent the interactive CREB-H (with PPAR?) and PPAR? (with CREB-H) protein signal intensities after normalization to ?-actin signal intensities. Ab, antibody; Ad, adenovirus.
Adapted from: Kim, H., et al. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82.
If you publish research with this product, please let us know so we can cite your paper.