This mouse IgG2bk monoclonal antibody [1F3-B3-E5] was generated against a KLH synthetic peptide encompassing Ras homology Growth-related (RhoG) amino acids 161-180 and is reactive against human, mouse and rat RhoG.
RhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in its place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.
From the laboratory of Martin A. Schwartz, PhD, University of Virginia.
Part of The Investigator's Annexe program.
|Molecular Weight:||21 kDa|
|Reactivity:||Human, mouse and rat|
|Immunogen:||KLH synthetic peptide encompassing amino acids RhoG 161-180|
|Purification Method:||Protein G Affinity Chromatography|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, ELISA|
Does not cross-react with Rac, Rho (A, B, C), or Cdc42
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