LF E687A (Anthrax Lethal Factor Mutant (LF E687A))

This is an inactive, catalytic site mutation (E687A) of lethal factor (LF). Note that this mutant has the native LF N-terminus, AGG.

Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.

From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ENH016-LT
LF E687A (Anthrax Lethal Factor Mutant (LF E687A))
100ug (3mg/mL) In stock
Regular Price:$269.00
On Sale:

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Specifications

Product Type: Protein
Name: E678A
Alternative Name(s): LF E687A
Source: Expressed in avirulent engineered B. anthracisstrain BH450
Format: Purified protein (liquid)
Purity: Hydroxyapatite Chromatography
Buffer: 10 mM HEPES pH 7.5, 0.50 mM EDTA
Concentration: 3mg/mL
Storage: -80C
Shipped: Dry ice

Provider
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
References
  1. Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.
  2. Vitale, G., Pellizzari, R., Recchi, C., Napolitani, G., Mock, M., and Montecucco, C. (1998) Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages. Biochem.Biophys.Res.Commun. 248, 706-711

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