LF E687A (Anthrax Lethal Factor Mutant (LF E687A))

This is an inactive, catalytic site mutation (E687A) of lethal factor (LF). Note that this mutant has the native LF N-terminus, AGG.

Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.

From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.

Read about how this product was utilized by Absolute Antibody to validate their, Recombinant Engineered Antibodies Against Anthrax.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
LF E687A (Anthrax Lethal Factor Mutant (LF E687A))
100ug (3mg/mL) In stock
Regular Price:$310.00
On Sale:

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Product Type: Protein
Name: E678A
Alternative Name(s): LF E687A
Accession ID: P15917
Source: Expressed in avirulent engineered B. anthracisstrain BH450
Format: Purified protein (liquid)
Purity: Hydroxyapatite Chromatography
Buffer: 10 mM HEPES pH 7.5, 0.50 mM EDTA
Concentration: 3mg/mL
Storage: -80C
Shipped: Dry ice

From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
  1. Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.
  2. Vitale, G., Pellizzari, R., Recchi, C., Napolitani, G., Mock, M., and Montecucco, C. (1998) Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages. Biochem.Biophys.Res.Commun. 248, 706-711

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