LFn-Linker-Cys (Anthrax Lethal Factor Mutant (LFn-Linker-Cys))
Lethal factor (LF) residues 1-254, followed by a short linker and a C-terminal Cysteine. This Cys provides a target for chemical conjugation of dyes, polypeptides, etc., through maleimide or iodoacetyl groups. The conjugated material will be internalized to the cytosol in the same way as LF or EF, provided the material has properties conducive to passage through the PA channel. LFn has the native N-terminus of AGG.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
Lethal factor (LF) residues 1-254, followed by a short linker and a C-terminal Cysteine. This Cys provides a target for chemical conjugation of dyes, polypeptides, etc., through maleimide or iodoacetyl groups. The conjugated material will be internalized to the cytosol in the same way as LF or EF, provided the material has properties conducive to passage through the PA channel. LFn has the native N-terminus of AGG.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
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| Product Type: | Protein |
| Name: | AGG-LFn-NLSLEELKD-GGGGSC |
| Alternative Name(s): | LFn-Linker-Cys |
| Accession ID: | P15917 |
| Source: | Expressed in avirulent engineered B. anthracisstrain BH460 |
| Format: | Purified protein (liquid) |
| Purity: | Q-Sepharose Ion Exchange Chromatography |
| Buffer: | 5 mM HEPES pH 7.5 and 0.5 mM EDTA, 0.25 mM DTT |
| Concentration: | 1.25mg/mL |
| Storage: | -80C |
| Shipped: | Dry ice |
- Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.
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