PA-U7-K562C (Anthrax Protective Mutant Antigen (PA-U7-K562C))

PA-U7 is the protective antigen (PA) mutant in which the furin site, aa 164-167, RKKR, is changed to PGG, as described above. The K563C substitution lies on the outside surface of protective antigen (PA), allowing for attachment of dyes, etc., using maleimide or iodoacetyl reactive groups. (The K562 residue here is the same as the K563 residue in native PA).

Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.

From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ENH010-LT
PA-U7-K562C (Anthrax Protective Mutant Antigen (PA-U7-K562C))
100ug (3.27mg/mL) In stock
Regular Price:$306.00
On Sale:

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Specifications

Product Type: Protein
Name: K562C; Furin site, aa 164-176, RKKR, is changed to PGG
Alternative Name(s): PA-U7-K562C
Strain: Expressed in avirulent engineered B. anthracisstrain BH460
Format: Purified protein (liquid)
Purity: Hydroxyapatite Chromatography
Buffer: 5 mM HEPES pH 7.5 and 0.5 mM EDTA, 0.20 mM DTT
Concentration: 3.27mg/mL
Storage: -80C
Shipped: Dry ice

Provider
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
References
  1. Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.

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