K563 is on the outside surface of protective antigen (PA) and of the PA oligomer. Modification of this residue does not interfere with oligomer formation or activity. The Cysteine added here provides a convenient target for attachment of dyes, etc., using maleimide or iodoacetyl reactive groups to react with the cysteine. Native PA contains no other cysteines.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
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|Expressed in avirulent engineered B. anthracisstrain BH450
|Purified protein (liquid)
|5 mM HEPES pH 7.5 and 0.5 mM EDTA, 0.25 mM DTT
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