Dominant negative F427A protective antigen (PA) mutant that fails to translocate LF and EF. The F427 residue is in the lumen of the channel, where the ring of 7 or 8 F residues constitutes the “Psi clamp”, which is necessary for translocation of LF and EF polypeptides.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
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|Alternative Name(s):||PA F427A|
|Strain:||Expressed in avirulent engineered B. anthracisstrain BH480|
|Format:||Purified protein (liquid)|
|Buffer:||10 mM HEPES pH 7.5, 0.50 mM EDTA|
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