PA-3M Cys (Anthrax Protective Mutant Antigen (PA-3M Cys))
PA-3M contains three mutated residues (D683A, L685E, Y688K) which are involved in the interaction of the protective antigen (PA) with its receptors, as described on a separate page. This protein also contains a cysteine on the C-terminus. This allows chemical conjugation of new targeting moieties, e.g., antibodies, growth factors, peptides, etc., so that the anthrax toxin proteins will be specific for defined cell types.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
PA-3M contains three mutated residues (D683A, L685E, Y688K) which are involved in the interaction of the protective antigen (PA) with its receptors, as described on a separate page. This protein also contains a cysteine on the C-terminus. This allows chemical conjugation of new targeting moieties, e.g., antibodies, growth factors, peptides, etc., so that the anthrax toxin proteins will be specific for defined cell types.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
In order to purchase this product, we require a Letter of Assurance form to be completed. Download form here: 
Kerafast Letter of Assurance
| Product Type: | Protein |
| Name: | D683A, L685E, Y688K, C-terminal Cys |
| Alternative Name(s): | PA3M Cys HTP2 |
| Accession ID: | P13423 |
| Strain: | Expressed in avirulent engineered B. anthracisstrain BH480 |
| Format: | Purified protein (liquid) |
| Purity: | Hydroxyapatite Chromatography |
| Buffer: | 5 mM Hepes pH 7.5, 0.50 mM EDTA, 1 mM DTT |
| Concentration: | 2.4mg/mL |
| Storage: | -80C |
| Shipped: | Dry ice |
- Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.
If you publish research with this product, please let us know so we can cite your paper.
