PA-3M Cys (Anthrax Protective Mutant Antigen (PA-3M Cys))

PA-3M contains three mutated residues (D683A, L685E, Y688K) which are involved in the interaction of the protective antigen (PA) with its receptors, as described on a separate page. This protein also contains a cysteine on the C-terminus. This allows chemical conjugation of new targeting moieties, e.g., antibodies, growth factors, peptides, etc., so that the anthrax toxin proteins will be specific for defined cell types.

Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.

From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ENH005-LT
PA-3M Cys (Anthrax Protective Mutant Antigen (PA-3M Cys))
100ug (2.4mg/mL) In stock
Regular Price:$306.00
On Sale:

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Specifications

Product Type: Protein
Name: D683A, L685E, Y688K, C-terminal Cys
Alternative Name(s): PA3M Cys HTP2
Strain: Expressed in avirulent engineered B. anthracisstrain BH480
Format: Purified protein (liquid)
Purity: Hydroxyapatite Chromatography
Buffer: 5 mM Hepes pH 7.5, 0.50 mM EDTA, 1 mM DTT
Concentration: 2.4mg/mL
Storage: -80C
Shipped: Dry ice

Provider
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
References
  1. Leppla SH. Production and purification of anthrax toxin. Methods Enzymol. 1988;165:103-16.

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