PA-3M contains three mutated residues (D683A, L685E, Y688K) which are involved in the interaction of the protective antigen (PA) with its receptors, as described on a separate page. This protein also contains a cysteine on the C-terminus. This allows chemical conjugation of new targeting moieties, e.g., antibodies, growth factors, peptides, etc., so that the anthrax toxin proteins will be specific for defined cell types.
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). Anthrax is caused by B. anthracis, a spore-forming, Gram positive, rod-shaped bacterium. The lethality of the disease is caused by the bacterium's two principal virulence factors: the polyglutamic acid capsule, which is anti-phagocytic, and the tripartite protein toxin, called anthrax toxin.
From the laboratory of Stephen H. Leppla, PhD, National Institute of Allergy and Infectious Diseases/NIH.
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|Name:||D683A, L685E, Y688K, C-terminal Cys|
|Alternative Name(s):||PA3M Cys HTP2|
|Strain:||Expressed in avirulent engineered B. anthracisstrain BH480|
|Format:||Purified protein (liquid)|
|Buffer:||5 mM Hepes pH 7.5, 0.50 mM EDTA, 1 mM DTT|
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