Human Mast Cell Line (LUVA)

Immortalized Human Mast Cell Line (LUVA) that is easily cultured without growth factors.

Highlights:

  • Fast growing and increased life span, compared to typical blood-derived CD34+ cells
  • Proliferate independent of exogenous stem cell factors
  • Contain granules and express high levels of mast cell cytokines, prostaglandins and leukotrienes

Mast cells exist in several types of tissues and play a role in allergy and anaphylaxis as a factor in inflammation response. They are also involved in wound healing and defense against pathogens. These cells express the high-affinity receptors that are specific to IgE, FceRI, on their surface, making IgE molecules cell-surface receptors for an antigen. When bound IgE molecules find a substrate, mast cells release inflammatory mediators like histamine.

From the laboratory of John W. Steinke, PhD, Larry C. Borish, MD, and S. Brandon Early, University of Virginia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EG1701-FP
Human Mast Cell Line (LUVA)
1 vial 4-6 weeks
Regular Price:$1,155.00
On Sale:

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Specifications

Product Type: Cell Line
Name: Human Mast Cell Line (LUVA)
Cell Type: Mast cell
Accession ID: CVCL_5G48
Source: Blood CD34+ derived
Organism: Human
Morphology: Suspension and adherent
Biosafety Level: BSL1
Subculturing: Cells can be split 1:2 – 1:20. Cells split 1:10 will be confluent in 3-4 days.
Growth Conditions: StemPro-34 SFM (500mL), L-glutamine (2mM final), 5mL Pen Strep (10,000 U/mL), 1mL Primocin (50mg). See PDF LUVA Protocol for instructions.
Cryopreservation: 90% Complete media, 10% DMSO (See PDF LUVA Protocol for instructions.)
Comments: The Steinke lab has noted a difference in how the cells are behaving that is different from the original publication. In the article, LUVA cells had high levels of expression of FceRI, however levels of around 5% by cell surface staining are currently being observed by the provider. Higher levels with intracellular staining are seen and very high mRNA expression for FceRI is detected. The reason for this difference is not clear.
Storage: Liquid nitrogen
Shipped: Dry ice

Documentation
Provider
From the laboratory of John W. Steinke, PhD, Larry C. Borish, MD, and S. Brandon Early, University of Virginia.
Comments
The Steinke lab has noted a difference in how the cells are behaving that is different from the original publication. In the article, LUVA cells had high levels of expression of FceRI, however levels of around 5% by cell surface staining are currently being observed by the provider. Higher levels with intracellular staining are seen and very high mRNA expression for FceRI is detected. The reason for this difference is not clear.
References
  1. Laidlaw, T.M., Steinke, J.W., Tiñana, A.M., Xing, W.,Lam, B.K., Paruchuri, S., Boyce, J.A., and Borish, L. Characterization of a novel human mast cell line that responds to stem cell factor and expresses functional FcεRI. Journal of Allergy and Clinical Immunology 127:815-822.e5 (2011)
  2. Kirshenbaum AS, Goff JP, Semere T, Foster B, Scott, LM and Metcalfe DD Demonstration that human mast cells arise from a progenitor cell population that is CD34+, c-kit+, and expresses aminopeptidase N (CD13) Blood 1999; 94,7: 2333-2342
  3. Johnson M, Alsaleh N, Mendoza RP, Persaud I, Bauer AK, Saba L, Brown JM. Genomic and transcriptomic comparison of allergen and silver nanoparticle-induced mast cell degranulation reveals novel non-immunoglobulin E mediated mechanisms. PLoS One. 2018 Mar 22;13(3):e0193499. View Article

If you publish research with this product, please let us know so we can cite your paper.

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