Anti-Integrin Beta 3 (GPIIIa, CD61), PSI Domain [AP-3] Antibody

This IgG1 mouse monoclonal (AP3) was generated against human platelets and is specific for PSI/Hybrid (amino acids 50-62) of human glycoprotein IIIa (GPIIIa; CD61).

Highlights:

  • Reacts with human glycoprotein IIIa
  • Suitable for non reduced Western Blot, Flow Cytometry, ELISA and Affinity Chromatography

Glycoprotein IIb/IIIa, also known as integrin aIIbß3 is an integrin complex found on platelets. The complex acts as a receptor for both fibrogen and von Willebrand factor and helps in platelet activation. Defects in glycoprotein IIb/IIIa cause Glanzmann's thrombasthenia, which is an abnormality in the platelets, or bleeding disorder.

From the laboratory of Richard H. Aster, MD, PhD, Blood Center of Wisconsin

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EBW106
Anti-Integrin Beta 3 (GPIIIa, CD61), PSI Domain [AP-3] Antibody
100ug In stock
Regular Price:$328.00
Specifications

Product Type: Antibody
Antigen: Platelets
Accession ID: P05106
Isotype: IgG1
Clonality: Monoclonal
Clone Name: AP3
Reactivity: Human
Immunogen: Human platelet
Species Immunized: Mouse
Epitope: PSI/Hybrid (amino acids 50-62)
Purification Method: Protein G
Method Used to Determine Concentration: A280
Buffer: 10 mM HEPES, 150 mM NaCl
Tested Applications: Flow Cytometry (0.02 mg/mL), ELISA capture (10ug/mL) and detection (10ug/mL)
Concentration: 2.1 mg/mL
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Richard H. Aster, MD, PhD, Blood Center of Wisconsin
References
  1. Pidard D, Montgomery RR, Bennett JS, Kunicki TJ. Interaction of AP-2, a monoclonal antibody specific for the human platelet glycoprotein IIb-IIIa complex, with intact platelets. J Biol Chem. 1983 Oct 25;258(20):12582-6.
  2. Peterson JA, Nelson TN, Kanack AJ, Aster RH. Fine specificity of drug-dependent antibodies reactive with a restricted domain of platelet GPIIIA. Blood. 2008 Feb 1;111(3):1234-9. Epub 2007 Oct 24.
  3. Ruiz C, Liu CY, Sun QH, Sigaud-Fiks M, Fressinaud E, Muller JY, Nurden P, Nurden AT, Newman PJ, Valentin N. A point mutation in the cysteine-rich domain of glycoprotein (GP) IIIa results in the expression of a GPIIb-IIIa (alphaIIbbeta3) integrin receptor locked in a high-affinity state and a Glanzmann thrombasthenia-like phenotype. Blood. 2001 Oct 15;98(8):2432-41.
  4. Honda S, Tomiyama Y, Pelletier AJ, Annis D, Honda Y, Orchekowski R, Ruggeri Z, Kunicki TJ. Topography of ligand-induced binding sites, including a novel cation-sensitive epitope (AP5) at the amino terminus, of the human integrin beta 3 subunit. J Biol Chem. 1995 May 19;270(20):11947-54.
  5. Faralli JA, Dimeo KD, Trane RM, Peters D. Absence of a secondary glucocorticoid response in C57BL/6J mice treated with topical dexamethasone. PLoS One. 2018 Mar 2;13(3):e0192665. View Article
  6. Faralli JA, Filla MS, Peters DM. Effect of αvβ3 Integrin Expression and Activity on Intraocular Pressure. Invest Ophthalmol Vis Sci. 2019 Apr 1;60(5):1776-1788. View Article

If you publish research with this product, please let us know so we can cite your paper.

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