UV Cleavable Biotin-Azide

The UV Cleavable Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.


  • Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
  • Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
  • Also available: 13C6-UV-Biotin-azide

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
UV Cleavable Biotin-Azide
10mg In stock
Regular Price:$355.00
On Sale:

Product Type: Small Molecule
Name: UV Cleavable Biotin-Azide
Chemical Formula: C38H51N7O9S
Source: Synthetic
Molecular Weight: 781.92
Format: Solid
Solubility: DMSO, MeOH
Photolysis: λ=365nm
Storage: -20C
Shipped: Ambient temperature

Cellular Click Chemistry Diagram
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
  1. Yang J, Tallman KA, Porter NA, Liebler DC. Quantitative chemoproteomics for site-specific analysis of protein alkylation by 4-hydroxy-2-nonenal in cells. Anal Chem. 2015 Mar 3;87(5):2535-41. doi: 10.1021/ac504685y. Epub 2015 Feb 9. PMID: 25654326; PMCID: PMC4350606.
  2. Alkylation Damage by Lipid Electrophiles Targets Functional Protein Systems. Codreanu, S. G.; Ullery, J. C.; Zhu, J.; Tallman, K. A.; Beavers, W. N.; Porter, N. A.; Marnett, L. J.; Zhang, B.; Liebler, D. C. Mol. Cell. Proteomics 2014, 13, 849.
  3. An Azido-Biotin Reagent for Use in the Isolation of Protein Adducts of Lipid-Derived Electrophiles by Streptavidin Catch and Photo-Release. Kim, H.-Y. H.; Tallman, K. A.; Liebler, D. C.; Porter, N. A. Mol. Cell. Proteomics 2009, 8, 2080.
  4. Yang J, Gupta V, Tallman KA, Porter NA, Carroll KS, Liebler DC. Global, in situ, site-specific analysis of protein S-sulfenylation. Nat Protoc. 2015 Jul;10(7):1022-37. View Article
  5. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article

If you publish research with this product, please let us know so we can cite your paper.