The Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.


  • Biotin-conjugate can be subsequently visualized using streptavidin Western techniques

For enrichment of the alkyne modified substrate, the UV Cleavable Biotin-Azide is recommended.

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
10mg In stock
Regular Price:$145.00
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Product Type: Small Molecule
Name: Biotin-Azide
Chemical Formula: C22H39N7O5
Source: Synthetic
Molecular Weight: 513.65
Format: Solid
Solubility: DMSO, MeOH
Storage: -20C
Shipped: Ambient temperature

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
  1. Assay of Protein and Peptide Adducts of Cholesterol Ozonolysis Products by Hydrophobic and Click Enrichment Methods. Windsor, K.; Genaro-Mattos, T. C.; Miyamoto, S.; Stec, D. F.; Kim, H.-Y. H.; Tallman, K. A.; Porter, N. A. Chem. Res. Toxicol. 2014, 27, 1757.
  2. Probing lipid-protein adduction with alkynyl surrogates: application to Smith-Lemli-Opitz syndrome. Windsor, K.; Genaro-Mattos, T. C.; Kim, H.-Y.; Liu, W.; Tallman, K. A.; Miyamoto, S.; Korade Z.; Porter, N. A. J. Lipid Res. 2013, 54, 2842.
  3. Identification of Protein Targets of 4-Hydroxynonenal Using Click Chemistry for ex Vivo Biotinylation of Azido and Alkynyl Derivatives. Vila, A.; Tallman, K. A.; Jacobs, A. T.; Liebler, D. C.; Porter, N. A.; Marnett, L. Chem. Res. Toxicol. 2008, 21, 432.
  4. Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J. Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol. 2017 Nov 16;24(11):1416-1427.e5. View Article
  5. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. View Article
  6. Heppner DE, Dustin CM, Liao C, Hristova M, Veith C, Little AC, Ahlers BA, White SL, Deng B, Lam YW, Li J, van der Vliet A. Direct cysteine sulfenylation drives activation of the Src kinase. Nat Commun. 2018 Oct 30;9(1):4522. View Article
  7. Dustin CM, Hristova M, Schiffers C, van der Vliet A. Proteomic Methods to Evaluate NOX-Mediated Redox Signaling. Methods Mol Biol. 2019;1982:497-515. View Article

If you publish research with this product, please let us know so we can cite your paper.