BMP responsive reporter cell line with dual luciferase reporter construct made using immortalized calvarial osteoblast cells isolated from tamoxifen inducible Bmp2/Bmp4 double conditional knockout mouse strain.
Bone Morphogenetic Proteins (BMPs) are a group of growth factors or cytokines which belongs to transforming growth factor beta (TGF-β) superfamily. There are at least 20 related BMPs known to contribute to embryogenesis and morphogenesis of various tissues and organs. BMPs are used to induce the formation of bone and cartilage during fracture healing and bone repair. BMP2 and BMP4 are two most osteogenic BMPs and have been demonstrated to induce osteogenic differentiation in a variety of cell types. Misregulation of the BMP signaling can leads to several developmental disorders and cancer, specifically in the progression of colon cancer. In contrast, over-activation of BMP signaling can leads to Barrett's oesophagus and several skeleton abnormalities [3, 4].
From the laboratory of Amitabha Bandyopadhyay, PhD, Indian Institute of Technology Kanpur.
|Product Type:||Cell Line|
|Growth Conditions:||DMEM + 10 % FBS (Pen/Strep @ concentration standard)|
|Subculturing:||Can be sub-cultured using standard cell culture protocols|
|Cryopreservation:||Growth medium containing 50% FBS +7% DMSO|
|Comments:||The reporter construct contains BRE driven Firefly Luciferase gene (FFLuc) and SV40 promoter/enhancer driven Renilla luciferase (RRLuc) gene, to serve as an internal normalization control for cell number as well as non-specific transcription activation|
Validation of Cell Line
BMP2 dependent FFLuc activity of BRITER cell line under four different conditions, namely 2BMP. 24-OHT, 2BMP, +4-OHT, +BMP, +4-OHT and +BMP, 24-OHT. BMP (bone morphogenetic protein 2) and OHT (hydroxytamoxifen).
Adapted from: Yadav PS., et al. PLoS One. 2012;7(5):e37134.
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