Non-Natural Promoter T7 Polymerase

A modified T7 RNA polymerase that orthogonally recognizes a non-natural promoter sequence: 5’-TAATACCGGTCACTATA, where the underlined residues differ from wild-type.

Highlights:

  • Does not recognize the wildtype promoter, nor does the wild type recognize the non natural promoter

From the laboratory of Andrew D. Ellington, PhD, University of Texas at Austin

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EUT001
Non-Natural Promoter T7 Polymerase
100ug (40 reactions) In stock
Regular Price:$204.00
Specifications

Product Type: Protein
Name: CGG-R12-KIR
Source: Escherichia coli
Molecular Weight: 100.142 kDa
Amino Acid Sequence: MRGSHHHHHHGSNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQFLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIESTGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIKTRVHIMFLGQFEMQPTINTNKDSEIDARKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA
Fusion Tag(s): 6xHis
Purity: > 98% (SDS-PAGE)
Buffer: 50 mM Tris, 50 mM NaCl, 1 mM DTT, 0.1% Triton-X 100, 0.1 mM EDTA, 50% glycerol
Concentration: 0.5mg/mL
Activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 ?l in 1 hour at 37°C in 1X RNA Polymerase Reaction Buffer
Comments: Promoter specificity change (CGG)
Storage: -20C
Shipped: Cold packs

Provider
From the laboratory of Andrew D. Ellington, PhD, University of Texas at Austin
References
  1. Ellefson JW, Meyer AJ, Hughes RA, Cannon JR, Brodbelt JS, Ellington AD. Directed evolution of genetic parts and circuits by compartmentalized partnered replication. Nat Biotechnol. 2014 Jan;32(1):97-101. doi: 10.1038/nbt.2714.
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