2,5 dibromohexanediamide (DBHDA)

Alkylating agent that mildly converts cysteine to dehydroalanine (Dha) residues.

Highlights:

  • Using a "tag-and-modify" approach, Dha can be used as a "handle" for further modifications
  • Various nucleophiles (e.g., thiols, thiophosphate, etc.) can react with Dha to afford a wide range of chemical modifications

From the laboratory of Benjamin G. Davis, PhD, University of Oxford.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EOX103
2,5 dibromohexanediamide (DBHDA)
50mg In stock
Regular Price:$536.00
Specifications

Product Type: Small Molecule
Name: 2,5 Dibromohexanediamide (DBHDA)
Chemical Formula: C6H10Br2N2O2
Molecular Weight: 299.91 kDa
Format: Off white solid
Purity: > 95% by 1HNMR Purity Information
Solubility: Soluble in DMSO and DMF, slightly soluble in methanol and H2O
Storage: Stable at room temperature. Store at -20C for long term storage
Shipped: Ambient temperature

Documentation

  Purity Information
PDF Product Data sheet

Protocol Notes

  1. Typically 200 equivalents of DBHDA are added to a protein solution in buffer.
  2. Shake at 37C for 1-4 hours.
  3. Desalting provides a pure chemically modified protein.
Provider
From the laboratory of Benjamin G. Davis, PhD, University of Oxford.
References
  1. Chalker JM, Gunnoo SB, Boutureira O, Gerstberger SC, Fernández-González M, Bernardes GJL Griffin L, Hailu H, Schofielda CJ, Davis BG. Methods for converting cysteine to dehydroalanine on peptides and proteins. Chem. Sci., 2011,2, 1666-1676.
  2. Nadal S, Raj R, Mohammed S, Davis BG. Synthetic post-translational modification of histones. Curr Opin Chem Biol. 2018 Feb 28;45:35-47. View Article
  3. Dadová J, Galan SR, Davis BG. Synthesis of modified proteins via functionalization of dehydroalanine. Curr Opin Chem Biol. 2018 Jun 15;46:71-81. View Article

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