U-251 MG Glioblastoma Cell Line

Permanent cell line U-251MG derived from a human malignant glioblastoma multiforme.


  • Continue to express glial fibrillary acidic protein (GFAP), over many cell passages
  • Expresses epidermal growth factor receptor (EGFR) receptors, for inhibition studies
  • Isolated from 75 year old male with astrocytoma, grade III-IV malignant tumor by explant technique

Astrocytomas are a type of cancer in the brain that originate in a particular kind of glial cells, called astrocytes. High-grade astrocytomas, also called glioblastoma multiforme are the most malignant of all brain tumors.

From the laboratory of Darell D. Bigner, MD, PhD, Duke University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
U-251 MG Glioblastoma Cell Line
1 vial 4-6 weeks
Regular Price:$780.00
On Sale:

Product Type: Cell Line
Name: U-251 MG
Cell Type: Glioblastoma
Accession ID: CVCL_0021
Source: Brain
Organism: Human
Morphology: Fibroblastic pattern
Biosafety Level: 2
Growth Conditions: Improved MEM Zinc Option +10% FBS, Penn/strep, amphotericin B
Subculturing: Split 1:2 or 1:3 every 3 days using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37C.
Cryopreservation: 90% (MEM Zinc Option with 20% FBS) + 10% DMSO
Storage: Liquid nitrogen
Shipped: Dry ice

From the laboratory of Darell D. Bigner, MD, PhD, Duke University.
  1. Bigner DD, Bigner SH, Pontén J, Westermark B, Mahaley MS, Ruoslahti E, Herschman H, Eng LF, Wikstrand CJ. Heterogeneity of Genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas. J Neuropathol Exp Neurol. 1981 May;40(3):201-29.
  2. Bigner SH, Bullard DE, Pegram CN, Wikstrand CJ, Bigner DD. Relationship of in vitro morphologic and growth characteristics of established human glioma-derived cell lines to their tumorigenicity in athymic nude mice. J Neuropathol Exp Neurol. 1981 Jul;40(4):390-409.
  3. Bullard DE, Schold SC Jr, Bigner SH, Bigner DD. Growth and chemotherapeutic response in athymic mice of tumors arising from human glioma-derived cell lines. J Neuropathol Exp Neurol. 1981 Jul;40(4):410-27.

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