Mouse Macrophage Cell Line (AA9) for PRRSV Propagation

Sialoadhesin expressing macrophage cell-line [AA9] for in-vitro propagation of porcine reproductive and respiratory syndrome virus (PRRSV).

Highlights:

  • Ideal cell line for the propagation of PRRSV in vitro
  • Transfected with porcine sialoadhesin - allows for permissive PRRSV replication

Porcine reproductive and respiratory syndrome virus (PRRSV) also known has blue-ear pig syndrome is an small enveloped RNA virus that causes disease in pigs. The virus effects reproductive failure in breeding stock and respiratory tract illness in young pigs. Sialoadhesin is the cell-surface protein on porcine alveolar macrophages (PAMs) that mediates the internalization of porcine reproductive and respiratory syndrome virus (PRRSV).

From the laboratory of Subramaniam (Sri) Srikumaran, PhD, Washington State University.

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EWU001
Mouse Macrophage Cell Line (AA9) for PRRSV Propagation
T25 flask Currently Unavailable
Regular Price:$799.00
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Specifications

Product Type: Cell Line
Name: AA9
Cell Type: Macrophage
Organism: Mouse
Accession ID: CVCL_0P26
Source: Ascites
Morphology: Macrophage
Biosafety Level: I
Growth Conditions: DMEM +10% FBS +2mM Glutamine and 500ug/mL G418
Subculturing: Scraping or trypsin can be used. Every 3-4 days into 1:4
Cryopreservation: 90% FBS + 10% DMSO
Format: Live cells (at 50% confluency) in a T25 flask
Storage: Liquid nitrogen
Shipped: Ambient temperature as live cells (at 50% confluency) in a T25 flask

Provider
From the laboratory of Subramaniam (Sri) Srikumaran, PhD, Washington State University.
References
  1. Shanthalingam S, Liu W, Snekvik K, Pattnaik A, Osorio FA, Srikumaran S. 2009. Transfection of porcine sialoadhesin into a murine macrophage cell-line renders it permissive for PRRSV replication. Veterinary Immunology and Immunopathology. 128:240-241
  2. Srikumaran S., Liu W., Shanthalingam S. Macrophage cell-lines for propagation of porcine reproductive and respiratory syndrome virus. US Patent 2010/0146653 A1, June 10, 2010.

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