Anti‐IQGAP2 [A2] Antibody

This IgG2a mouse monoclonal antibody was generated against purified, recombinant, N-terminally his-tagged human IQGAP2 (isoform 1), and is reactive towards human and mouse IQGAP2.

Highlights:

  • Reacts with the N-terminus of IQGAP2 (amino acids 1-528)
  • Specific for IQGAP2 - Does not recognize IQGAP1
  • Recommended for Western Blot and Immunofluorescence applications

IQ motif containing GTPase activating protein (IQGAP) is a scaffolding protein that interacts with cytoskeleton, cell adhesion molecules, and with signaling molecules to regulate cell morphology and motility. The protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain, and one WW domain. It has been known that IQGAP2 binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. IQGAP2 is found in the liver, stomach, and platelets and is over 50% identical to IQGAP1, but appears to have a drastically divergent function in terms of pathology.

From the laboratory of George S. Bloom, PhD and Eva Ho-Man Kan, University of Virginia

Catalog Number Product Size AVAILABILITY Price Qty
EG1601
Anti‐IQGAP2 [A2] Antibody
100ug In stock
Regular Price:$364.00
Specifications

Product Type: Antibody
Name: Anti-IQGAP2 [A2] Antibody
Accession ID: NP 006624; NP001272389.1 (isoform 1 and 2, respectively)
Molecular Weight: 181 kDa (isoform 1), 174 kDa (isoform 2)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: A2
Reactivity: Human, Mouse
Immunogen: Purified, recombinant, N-terminally his-tagged human IQGAP2 (isoform 1)
Species Immunized: Mouse
Epitope: N-terminus (amino acids 1-528)
Purification Method: Protein G Affinity Chromatography
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB, IF
Storage: -20C
Shipped: Cold packs

Data

Immunofluorescence

CHO-K1 (Chinese hamster ovary) cells transfected to express the indicated fluorescently tagged fusion proteins derived from human IQGAP2 or IQGAP1 were stained by immunofluorescence with mouse monoclonal anti-IQGAP2 (clone A2) followed by AlexaFluor 594-goat anti-mouse IgG. Note that anti-IQGAP2 labeled the N-terminal 1/3 (amino acids 1-528) and full length (FL) versions of IQGAP2, but did not label FL IQGAP1.

Western Blot

An unfractionated homogenate of AG5 human gastric carcinoma cells was labeled with anti-IQGAP2 (clone A2). A single band in the MW range of full length IQGAP2 (~174,000) was labeled.

Protein Expression in Cells

Protein expression of IQGAP2 in revertant and susceptible cells. A) S7 and R7 cells in presence and absence of RA were labelled with mAb anti-IQGAP2 [A2]. B) The number of IQGAP2-positive R7 (grey) and S7 (black) cells analysed using Volocity analysis software.  Scale bar: 20um.

Adapted from: Marbiah M, et al. EMBO. J. 2014 Jul 17;33(14):1527-47.

Provider
From the laboratory of George S. Bloom, PhD and Eva Ho-Man Kan, University of Virginia
References
  1. Marbiah M, Harvey A, West B, Louzolo A, Banerjee P, Alden J, Grigoriadis A, Hummerich H, Kan H-M, Cai Y, Bloom GS, Parmjit J, Collinge J and Klöhn P-C. Identification of a gene regulatory network associated with prion replication. EMBO. J. 2014 Jul 17;33(14):1527-47.

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