FITC-trehalose selectively labels live mycobacteria in vitro and in vivo, allowing for downstream fluorescent imaging of Mycobacterium tuberculosis in culture and in infected macrophages.
Tuberculosis is an infection that has plagued mankind for millennia, and remains a leading cause of death worldwide. A substantial obstacle to the development of new diagnostics, drugs and vaccines is the lack of tuberculosis-specific probes that can be used to rapidly assess infection and monitor response to treatment.
From the laboratory of Benjamin G. Davis, PhD, University of Oxford.
|Product Type:||Small Molecule|
|Purity:||95+% (NMR, HPLC)|
|Solubility:||Soluble in MeOH and water|
[α]D = 72.2 (c = 0.18, MeOH); 1H NMR (500 MHz, D2O): δ 1.48 (3 H, s, C-1’), 3.26 (1 H, d, J3,4 = 9.8 Hz, H-3’), 3.49 (3 H, m, H4’, H-5’, H-4), 3.74 (6 H, m, H5, H-6’, H-6a, H-6b, H-7a’, H-7b’), 4.00 (1 H, at, J2,3 = J3,4 = 9.8 Hz, H-3), 4.41 (1 H, dd, J2,3 = 10.9 Hz,
|Storage:||-20C, protect from light|
Labeling of individual bacilli in vitro and within infected macrophages
(a) H37Rv M. tuberculosis are labeled with FITC-trehalose and show signification accumulation of probe at the bacterial poles and membrane. (b) FITC-trehalose labeling of the RFP-expressing strain of H37Rv. (c) A merge of the FITC-trehalose and the RFP channels. (d) The mean fluorescence of poles and midsections of H37Rv M. tuberculosis, labeled with FITC-trehalose, are quantified and show statistically significant differential labeling. RFU, relative fluorescence units.
Adapted from: Backus, KM et al. Nat. Chem. Biol. 7, 228–235 (2011).
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