Anti-Prothrombin [29J3-62] Antibody

This mouse monoclonal antibody (clone 29J3-62) is specific for human prothrombin. 29J3-62 has a high affinity for both soluble prothrombin and prothrombin bound to phospholipid or apoptotic cells, and represents a prototype for a phospholipid-independent antiphospholipid antibody.


  • Shows high affinity for soluble prothrombin
  • Also reacts with prothrombin bound to phospholipid or apoptotic cells
  • Shown to enhance binding of prothrombin to apoptotic cells
  • Acts as an antiphospholipid antibody that can bind to prothrombin in the absence or presence of phospholipid
  • Possesses lupus anticoagulant activity - useful as positive control in in vitro coagulation assays

?Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (prothrombin and factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of prothrombin (factor II) leads to formation of thrombin, which activates factors V, VII, VIII and XIII in complex with thrombomodulin and protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.

From the laboratory of Joyce Rauch, PhD, McGill University.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-Prothrombin [29J3-62] Antibody
100ug In stock
Regular Price:$255.00
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Product Type: Antibody
Antigen: Prothrombin (Coagulation factor II)
Accession ID: P00734
Isotype: 70.037 kDa (theoretical);IgG1k
Clonality: Monoclonal
Clone Name: 29J3-62
Reactivity: Human
Immunogen: Human prothrombin
Species Immunized: Mouse
Purification Method: Protein G
Buffer: PBS, pH 7.4
Tested Applications: ELISA (1:5000 or 0.2 ug/mL)
Concentration: 1 mg/mL
Storage: -20C
Shipped: Cold packs

From the laboratory of Joyce Rauch, PhD, McGill University.
  1. D'Agnillo P, Levine JS, Subang R, Rauch J. Prothrombin binds to the surface of apoptotic, but not viable, cells and serves as a target of lupus anticoagulant autoantibodies. J Immunol. 2003 Mar 15;170(6):3408-22.
  2. Rauch J, D'Agnillo P, Subang R, Levine JS. Anti-phospholipid antibodies (aPL) and apoptosis: prothrombin-dependent aPL as a paradigm for phospholipid-dependent interactions with apoptotic cells. Thromb Res. 2004; 114(5-6): 371-382.
  3. Roggenbuck D, Borghi MO, Somma V, Büttner T, Schierack P, Hanack K, Grossi C, Bodio C, Macor P, von Landenberg P, Boccellato F, Mahler M, Meroni PL. Antiphospholipid antibodies detected by line immunoassay differentiate amongpatients with antiphospholipid syndrome, with infections and asymptomatic carriers. Arthritis Res Ther. 2016 May 21;18(1):111. View Article

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