This mouse IgG2 monoclonal antibody was generated against DNA coding for murine IL-15 in a baculovirus vector. The antibody recognizes protein p25 an occlusion-derived virus envelope protein (Autographa californica nucleopolyhedrovirus) on the baculovirus surface.
The baculoviruses are a family of large rod-shaped viruses that can be divided to two genera: nucleopolyhedroviruses (NPV) and granuloviruses (GV). While GVs contain only one nucleocapsid per envelope, NPVs contain either single (SNPV) or multiple (MNPV) nucleocapsids per envelope. The enveloped virions are further occluded in granulin matrix in GVs and polyhedrin for NPVs. Moreover, GV have only single virion per granulin occlusion body while polyhedra can contain multiple embedded virions.
From the laboratory of John W. Kappler, PhD and Philippa Marrack, PhD, National Jewish Health.
Part of The Investigator's Annexe program.
|Antigen:||Baculovirus Occlusion-Derived Virus Envelope Protein E25 (p25)|
|Molecular Weight:||25 kDa|
|Reactivity:||Reacts with baculovirus from Autographa californica|
|Immunogen:||Sf9 cells previously transfected with DNA coding for murine IL-15 in a baculovirus vector|
|Species Immunized:||Armenian hamster|
|Purification Method:||Protein G column|
|Buffer:||0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, Cell surface staining|
Western Blot: Approximately 1x108 baculovirus particles per lane of a 12% SDS Mini-gel with 2-mercaptoethanol.Transfer to Hybond ECL Nitrocellulose MembraneBiotinylated MKBac1-65.2 at 14ug/mL, detection with Streptavidin-HRP. Band is very strong at 25kd.
Cell surface staining: SF9 insect cells infected at MOI of ~1 to 3. Staining of cells on Day 3 post-infection with direct PE-MKBac1-65.2 conjugate, at 40ug/mL to 5ug/mL in 2-fold dilutions. Analysis on BD FacsCalibur shows a biphasic staining pattern with both intermediate and high staining.
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