This rabbit IgG polyclonal antibody was generated against the GST-ADAM17-cytoplasmic tail and is specific for the cytoplasmic domain of mouse ADAM17.
The ADAM (A Disintegrin And Metalloprotease Domain) family is a unique group of proteins that has the ability to cleave or shed extracellular portions of transmembrane proteins. In addition, ADAMs are involved in cell-cell and cell-matrix interactions (e.g., fertilization, muscle development and neurogenesis).
ADAM17 (also referred to as Tumor Necrosis Factor Convertase, TACE) is involved in the processing of tumor necrosis factor alpha (TNF-a) at the surface of the cell, and is also required for shedding several ligands of the epidermal growth factor receptor and many other membrane proteins.
From the laboratory of Carl P. Blobel, MD, PhD, Hospital for Special Surgery.
Part of The Investigator's Annexe program.
|Antigen:||GST-ADAM17 Cytoplasmc Domain|
|Molecular Weight:||~100 kDa (mature form), ~120 kDa (pro-form)|
|Immunogen:||GST-cyto corresponding to the cytoplasmic domain of mouse ADAM17|
|Purification Method:||Serum, not purified|
|Tested Applications:||WB (1:1000)|
Western Blot Analysis
Antibodies against: pro-domain fused to GST (α-PRO), cytoplasmic domain fused to GST (α-CYTO).
Adapted from: Schlöndorff J, et al. JBiochem J. 2000, 347, 131-138.
Cell lysis buffer must contain 5 mM 1,10 Phenanthroline to prevent autodegradation of the mature form of ADAM17, which is not prevented by EDTA or typical protease inhibitor cocktails (Schlöndorff et al., Biochem J. 2000, 347, 131-138).
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